Anti-Human ROBO1 Recombinant Antibody (A7241A)
CAT#: TAB-0281CL
The anti-ROBO1 antibody (A7241A) specifically binds to roundabout homolog 1, which has be identified as a molecule regulating the midline crossing of anxon and has been reported to work as a receptor for the Slit protein in subsequent studies. The specific antibody can be used for the diagnosis and treatment studies of cancer and a method for monitoring progression of hepatitis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52968/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169855-ROBO1
❮
❯
Specifications
- Host Species
- Mouse
- Specificity
- Human
- Clone
- A7241A
- Applications
- IB, IHC
- Related Disease
- Hepatitis
Applications
- Application Notes
- This antibody has been reported for use in Immunoblot and Immunofluorescence and Immunohistochemistry.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for TAB-0281CL. Click the button above to contact us or submit your feedback about this product.
Excellent for ROBO1
I used the anti-ROBO1 recombinant antibody in both immunoblotting and immunohistochemistry. The antibody clearly detects human ROBO1 with high specificity. Its detailed quality control measures and reliable performance have made it a trusted reagent for studying axon guidance and related protein expression in my experiments.
Consistent Detection
The anti-ROBO1 antibody provides consistent detection of the target protein in IB and IHC applications. Its high purity and reliable antibody performance support accurate assessment of ROBO1 expression. I appreciate the clear staining results and reproducible data it delivers in my research.
Q&As
-
Is this antibody validated for IHC and IB applications?
A: Yes, the anti-ROBO1 recombinant antibody has been validated for both immunoblot and immunohistochemistry applications. It specifically binds human ROBO1 with excellent clarity, supporting detailed analyses of protein expression in neural and cancer cell studies.
-
How reliable is the product for detecting ROBO1?
A: The antibody features high specificity and affinity towards human ROBO1. Its rigorous validation and high purity give researchers confidence in obtaining clear immunoblot and IHC results, making it a dependable choice for investigating ROBO1-related pathways in vitro.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone A7241A"
- CAT
- Product Name
See other products for "ROBO1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-346 | Mouse Anti-ROBO1 Recombinant Antibody (PABL-346) | WB, ELISA, FuncS | Mouse IgG2a kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-346 | Mouse Anti-ROBO1 Recombinant Antibody; scFv Fragment (PSBL-346) | WB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-346 | Mouse Anti-ROBO1 Recombinant Antibody; Fab Fragment (PFBL-346) | WB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0282CL | Anti-Human ROBO1 Recombinant Antibody (B2318C (binds to extracellular region of ROBO1)) | Cyt, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0281CL-S(P) | Anti-Human ROBO1 Recombinant Antibody scFv Fragment (A7241A) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0282CL-S(P) | Anti-Human ROBO1 Recombinant Antibody scFv Fragment (B2318C (binds to extracellular region of ROBO1)) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0281CL-F(E) | Anti-Human ROBO1 Recombinant Antibody Fab Fragment (A7241A) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0282CL-F(E) | Anti-Human ROBO1 Recombinant Antibody Fab Fragment (B2318C (binds to extracellular region of ROBO1)) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1275MZ | Recombinant Mouse Anti-Human ROBO1 Antibody (clone 2G6) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2166-CN | Rabbit Anti-ROBO1 Polyclonal Antibody (MRO-2166-CN) | IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0064 | Mouse Anti-ROBO1 Recombinant Antibody (PABJ-0064) | ELISA, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0064-S(P) | Mouse Anti-ROBO1 Recombinant Antibody; scFv Fragment (PABJ-0064-S(P)) | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0064-F(E) | Mouse Anti-ROBO1 Recombinant Antibody; Fab Fragment (PABJ-0064-F(E)) | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY52 | Human Anti-ROBO1 scFv-Fc Chimera (VS-0125-FY52) | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC350 | Recombinant Anti-ROBO1 Vesicular Antibody, EV Displayed (VS-0425-YC350) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6171 | Anti-ROBO1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC133 | Anti-ROBO1 Antibody Prodrug, Protease Activated (VS-1025-YC133) | ISZ, Cyt, FuncS |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
-2.png)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, Inhib, FC, ELISA
Application: Block, IP, IF, FC
Application: Neut, ELISA, WB, SPR, IF, EM
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
