Anti-Human RYK Recombinant Antibody (RWD1)
CAT#: TAB-375CT
Recombinant antibody specifically binds to the WIF domain of human RYK. This antibody modulates RYK-associated activity, which includes RYK mediated signal transduction activity and modulation of the interaction of Wnts with RYK and, preferably, modulates Wnt induced signaling. It inhibit the binding of Wnt5a and inhibit Wnt induced phosphorylation of Dishevelled (Dvl) 2 and/or Dvl3 proteins.
Published Data
Gene Expression
WB
Figure 1 Characterization of the human inhibitory anti-RYK IgG1k antibody (RWD1).
Anti-FLAG IPs were immunoblotted (IB) with an anti-Myc antibody to detect Wnt binding to hRYK.Fc. Molecular mass standards are shown at left in kDa.
Halford, M. M., Macheda, M. L., Parish, C. L., Takano, E. A., Fox, S., Layton, D., ... & Stacker, S. A. (2013). A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. PLoS One, 8(9), e75447.
IHC
Figure 2 Example of IHC on a tumor from a formalin-fixed and paraffin-embedded human breast cancer tissue microarray stained with bRWD1.
Open arrow, RYK on a cancer cell; filled arrow, RYK on tumor stroma; arrowhead, RYK-positive cancer cell nucleus; b, biotinylated on N-glycan chains. Bar represents 50 mm.
Halford, M. M., Macheda, M. L., Parish, C. L., Takano, E. A., Fox, S., Layton, D., ... & Stacker, S. A. (2013). A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. PLoS One, 8(9), e75447.
ELISA
Figure 3 ELISA analysis of immobilized RWD1 probed with hRYK.Fc.
Increased hRYK.Fc binding to the antibody was observed with higher concentrations of either immobilized RWD1. Results represent the mean6standard deviation of two or three independent experiments. IgG, human IgG; OD, optical density.
Halford, M. M., Macheda, M. L., Parish, C. L., Takano, E. A., Fox, S., Layton, D., ... & Stacker, S. A. (2013). A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. PLoS One, 8(9), e75447.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75430/1614_E12_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45503/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34117/71467_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34117/71467_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34117/71467_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34117/71467_A_5_6.jpg
Normal Tissue
Figure 7 Endometrium
Cells in endometrial stroma Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34117/71467_B_1_3.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34117/71467_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163785-RYK
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Specifications
- Immunogen
- H-RYK-FLAG protein
- Host Species
- Human
- Derivation
- Human phage display library
- Type
- Human antibody
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- RWD1
- Applications
- WB, IHC, ELISA
- Related Disease
- Lung, colon, prostate, breast, and skin cancer
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunohistochemistry and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- RYK; receptor-like tyrosine kinase; JTK5; RYK1; JTK5A; D3S3195; tyrosine-protein kinase RYK; hydroxyaryl-protein kinase; JTK5A protein tyrosine kinase; anti-RYK
- Gene ID
- 6259
- UniProt ID
- A0A087WUK1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-375CT-S(P) | Anti-Human RYK Recombinant Antibody scFv Fragment (RWD1) | WB, ELISA, BL. IHC, IP, FC | Human antibody |
| TAB-375CT-F(E) | Anti-Human RYK Recombinant Antibody Fab Fragment (RWD1) | WB, ELISA, BL. IHC, IP, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2415CT | Recombinant Mouse anti-Human RYK Monoclonal antibody (FQS4388) | WB | |
| HPAB-M0628-YC | Mouse Anti-RYK Recombinant Antibody (HPAB-M0628-YC) | Inhib | Mouse IgG |
| VS3-CJ887 | Mouse Anti-RYK Recombinant Antibody (VS3-CJ887) | WB, FC | Mouse IgG1, κ |
| VS3-FY1945 | Rabbit Anti-RYK Recombinant Antibody (clone R03-4E1) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| VS3-FY2867 | Mouse Anti-RYK Recombinant Antibody (VS3-FY2867) | WB, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3148 | Hi-Affi™ Recombinant Rabbit Anti-RYK Monoclonal Antibody (DS3148AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0628-YC-S(P) | Mouse Anti-RYK Recombinant Antibody; scFv Fragment (HPAB-M0628-YC-S(P)) | Inhib | Mouse scFv |
| HPAB-M0629-YC-S(P) | Mouse Anti-RYK Recombinant Antibody; scFv Fragment (HPAB-M0629-YC-S(P)) | Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0628-YC-F(E) | Mouse Anti-RYK Recombinant Antibody; Fab Fragment (HPAB-M0628-YC-F(E)) | Inhib | Mouse Fab |
| HPAB-M0629-YC-F(E) | Mouse Anti-RYK Recombinant Antibody; Fab Fragment (HPAB-M0629-YC-F(E)) | Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC472 | Recombinant Anti-RYK Vesicular Antibody, EV Displayed (VS-0425-YC472) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6289 | Anti-RYK Immunohistochemistry Kit | IHC |
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