Anti-Human RYK Recombinant Antibody (RWD1) (CAT#: TAB-375CT)
Recombinant antibody specifically binds to the WIF domain of human RYK. This antibody modulates RYK-associated activity, which includes RYK mediated signal transduction activity and modulation of the interaction of Wnts with RYK and, preferably, modulates Wnt induced signaling. It inhibit the binding of Wnt5a and inhibit Wnt induced phosphorylation of Dishevelled (Dvl) 2 and/or Dvl3 proteins.
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WB
Figure 1 Characterization of the human inhibitory anti-RYK IgG1k antibody (RWD1).
Anti-FLAG IPs were immunoblotted (IB) with an anti-Myc antibody to detect Wnt binding to hRYK.Fc. Molecular mass standards are shown at left in kDa.
Halford, M. M., Macheda, M. L., Parish, C. L., Takano, E. A., Fox, S., Layton, D., ... & Stacker, S. A. (2013). A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. PLoS One, 8(9), e75447.
IHC
Figure 2 Example of IHC on a tumor from a formalin-fixed and paraffin-embedded human breast cancer tissue microarray stained with bRWD1.
Open arrow, RYK on a cancer cell; filled arrow, RYK on tumor stroma; arrowhead, RYK-positive cancer cell nucleus; b, biotinylated on N-glycan chains. Bar represents 50 mm.
Halford, M. M., Macheda, M. L., Parish, C. L., Takano, E. A., Fox, S., Layton, D., ... & Stacker, S. A. (2013). A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. PLoS One, 8(9), e75447.
ELISA
Figure 3 ELISA analysis of immobilized RWD1 probed with hRYK.Fc.
Increased hRYK.Fc binding to the antibody was observed with higher concentrations of either immobilized RWD1. Results represent the mean6standard deviation of two or three independent experiments. IgG, human IgG; OD, optical density.
Halford, M. M., Macheda, M. L., Parish, C. L., Takano, E. A., Fox, S., Layton, D., ... & Stacker, S. A. (2013). A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. PLoS One, 8(9), e75447.
Specifications
- Immunogen
- H-RYK-FLAG protein
- Host Species
- Human
- Derivation
- Human phage display library
- Type
- Human antibody
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- RWD1
- Applications
- WB, IHC, ELISA
- Related Disease
- Lung, colon, prostate, breast, and skin cancer
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunohistochemistry and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- RYK; receptor-like tyrosine kinase; JTK5; RYK1; JTK5A; D3S3195; tyrosine-protein kinase RYK; hydroxyaryl-protein kinase; JTK5A protein tyrosine kinase; anti-RYK
- Gene ID
- 6259
- UniProt ID
- A0A087WUK1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-375CT-S(P) | Anti-Human RYK Recombinant Antibody scFv Fragment (RWD1) | WB, ELISA, BL. IHC, IP, FC | Human antibody |
| TAB-375CT-F(E) | Anti-Human RYK Recombinant Antibody Fab Fragment (RWD1) | WB, ELISA, BL. IHC, IP, FC | Human antibody |
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For Research Use Only. Not For Clinical Use.
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