Recombinant Mouse Antibody (BC-22) is capable of binding to Rabbit CTNNB1 (residue 19-44), expressed in Chinese Hamster Ovary cells (CHO). The peptide's epitope is predominantly located in the large bend and in the N-terminal segment, implicating bidentate association.
Figure 1 HGF enhances anti-Fas-induced apoptosis likely at the level significantly reduced in the presence of HGF, of caspase-8 activation.
A: Jurkat cells deficient in caspase-8 (JB6) and its caspase-8 reconstituted subline (BC-22) were treated with CH11 anti-Fas antibody (100 ng/ml) with or without HGF (0.5 mg/ml) for 12 h. Cell death was assessed by trypan blue staining (mean ± s.d.). B: Different Jurkat cell lines as indicated were treated with medium (black column), etoposide (20 mM, gray column) or etoposide plus 0.5 mg/ml of HGF (white column) for 12 h and analyzed for cell death by the trypan blue assay (mean W s.d.).
Zhao, Y., DiFrancesca, D., Wang, X. U. E., Zarnegar, R., Michalopoulos, G. K., & Yin, X. M. (2007). Promotion of Fas‐mediated apoptosis in Type II cells by high doses of hepatocyte growth factor bypasses the mitochondrial requirement. Journal of cellular physiology, 213(2), 556-563.
Figure 2 Binding of mAb BC-22 to the phosphorylated P-b-Cat19–44 peptide
Megy, S., Bertho, G., Gharbi-Benarous, J., Baleux, F., Benarous, R., & Girault, J. P. (2006). STD and TRNOESY NMR studies for the epitope mapping of the phosphorylation motif of the oncogenic protein β-catenin recognized by a selective monoclonal antibody. FEBS letters, 580(22), 5411-5422.
Figure 3 Epitope mapping of the P-β-Cat19–44 peptide in the presence of mAb, for a peptide:antibody ratio of 100:1.
Expansion of the region containing resonances of the amide protons: Reference 1D ¹ H spectrum (in black) of the P-β-Cat19–44 peptide in association with the mAb antibody and 1D ¹ H STD NMR spectrum (in red) of the P-β-Cat19–44 peptide in association with the mAb antibody, showing enhancements of resonances of protons making close contacts with the antibody-combining site. STD values are obtained after peak picking intensities compared to the 1D with the exact values of chemical shifts of the HN (in bold: amino acids with intense relative STD, and in italic: amino acids with weak relative STD). Variations of the chemical shifts between the HN are equal to or higher than 0.01 ppm. There are only Ala20 and Ser23, which pose problem since they leave together to 8.52 ppm. The value given can thus correspond only to the average of relative STD value found for each one.
Megy, S., Bertho, G., Gharbi-Benarous, J., Baleux, F., Benarous, R., & Girault, J. P. (2006). STD and TRNOESY NMR studies for the epitope mapping of the phosphorylation motif of the oncogenic protein β-catenin recognized by a selective monoclonal antibody. FEBS letters, 580(22), 5411-5422.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-247 | Recombinant Anti-human CTNNB1 Antibody | WB, FC, FuncS | IgG |
MHH-247 | Recombinant Human Anti-human CTNNB1 Antibody | ELISA, FuncS | IgG |
MRO-1692-CN | Rabbit Anti-CTNNB1 Polyclonal Antibody (MRO-1692-CN) | WB, IHC, FC | Rabbit IgG |
MRO-2294-CN | Rabbit Anti-CTNNB1 Polyclonal Antibody (MRO-2294-CN) | WB, IF, IHC | Rabbit IgG |
VS3-FY203 | Recombinant Mouse Anti-CTNNB1 Antibody (VS3-FY203) | WB, IHC | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-247-F(E) | Recombinant Anti-human CTNNB1 Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
MHH-247-F(E) | Recombinant Human Anti-human CTNNB1 Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
PSBZ-140 | Mouse Anti-CTNNB1 Recombinant Antibody (clone BC-22); scFv Fragment | ELISA, Block | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-247-S(P) | Recombinant Anti-human CTNNB1 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
MHH-247-S(P) | Recombinant Human Anti-human CTNNB1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
PFBZ-140 | Mouse Anti-CTNNB1 Recombinant Antibody (clone BC-22); Fab Fragment | ELISA, Block | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-049 | Recombinant Anti-Mouse Ctnnb1 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0859 | Hi-Affi™ Rabbit Anti-CTNNB1 Recombinant Antibody (clone DS859AB) | WB | Rabbit IgG |
MOR-0039-FY | Rabbit Anti-CTNNB1 Recombinant Antibody (clone AFY0010) | ICC, IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-008CN | Mouse Anti-CTNNB1 Recombinant Antibody (clone 13C6) | ELISA, IHC | Mouse IgG1 |
NS-008CN-F(E) | Mouse Anti-CTNNB1 Recombinant Antibody (clone 13C6); Fab Fragment | ELISA, IHC | Mouse Fab |
NS-008CN-S(P) | Mouse Anti-CTNNB1 Recombinant Antibody (clone 13C6); scFv Fragment | ELISA, IHC | Mouse scFv |
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