Human Anti-MOG Recombinant Antibody (clone 8-18C5) (CAT#: PABC-109)

Recombinant Chimeric (Human/Mouse) Antibody (8-18C5) is capable of binding to Rat MOG (myelin oligodendrocyte glycoprotein).


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WB

Figure 1 Western blot analysis of MOG expression in cell lysates from LN18MOG and LN18Ctr cells in nonreducing condition.

Figure 1 Western blot analysis of MOG expression in cell lysates from LN18MOG and LN18Ctr cells in nonreducing condition.

The 8-18C5 mAb stains two proteins corresponding to monomeric and dimeric MOG.

Zhou, D., Srivastava, R., Nessler, S., Grummel, V., Sommer, N., Brück, W., ... & Hemmer, B. (2006). Identification of a pathogenic antibody response to native myelin oligodendrocyte glycoprotein in multiple sclerosis. Proceedings of the National Academy of Sciences, 103(50), 19057-19062.

ICC

Figure 2 Recombinant expression of native MOG to determine antibody binding.

Figure 2 Recombinant expression of native MOG to determine antibody binding.

Immunocytochemistry of LN18MOG (×200) by 8-18C5 mAb.

Zhou, D., Srivastava, R., Nessler, S., Grummel, V., Sommer, N., Brück, W., ... & Hemmer, B. (2006). Identification of a pathogenic antibody response to native myelin oligodendrocyte glycoprotein in multiple sclerosis. Proceedings of the National Academy of Sciences, 103(50), 19057-19062.

IF

Figure 3 Human antibodies to native MOG bind to intact myelin.

Figure 3 Human antibodies to native MOG bind to intact myelin.

Rat brain slices were stained with 8–18C5 mAb. Staining was visualized by an anti-IgG antibody labeled with Alexa Fluor 488. Magnification: ×100

Zhou, D., Srivastava, R., Nessler, S., Grummel, V., Sommer, N., Brück, W., ... & Hemmer, B. (2006). Identification of a pathogenic antibody response to native myelin oligodendrocyte glycoprotein in multiple sclerosis. Proceedings of the National Academy of Sciences, 103(50), 19057-19062.

IHC

Figure 4 Human antinative MOG antibodies induce demyelination and axonal damage in rat EAE.

Figure 4 Human antinative MOG antibodies induce demyelination and axonal damage in rat EAE.

EAE was induced in Lewis rats, and different sera or 8–18C5 mAb were injected intravenously.

Zhou, D., Srivastava, R., Nessler, S., Grummel, V., Sommer, N., Brück, W., ... & Hemmer, B. (2006). Identification of a pathogenic antibody response to native myelin oligodendrocyte glycoprotein in multiple sclerosis. Proceedings of the National Academy of Sciences, 103(50), 19057-19062.

Inhib

Figure 5 Human antibodies to native MOG induce cell death of MOG-expressing target cells.

Figure 5 Human antibodies to native MOG induce cell death of MOG-expressing target cells.

LN18MOG (gray bars) and LN18Ctr (black bars) cells were incubated with anti-MOG antibody-positive and -negative MS sera. 8-18C5 mAb supplemented by serum from an antibody-negative patient was used as control.

Zhou, D., Srivastava, R., Nessler, S., Grummel, V., Sommer, N., Brück, W., ... & Hemmer, B. (2006). Identification of a pathogenic antibody response to native myelin oligodendrocyte glycoprotein in multiple sclerosis. Proceedings of the National Academy of Sciences, 103(50), 19057-19062.


Specifications

  • Immunogen
  • Rat myelin oligodendrocyte glycoprotein
  • Host Species
  • Human
  • Derivation
  • Chimeric (mouse/human)
  • Type
  • Chimeric (mouse/human) IgG1
  • Specificity
  • Rat MOG
  • Species Reactivity
  • Rat
  • Clone
  • 8-18C5
  • Applications
  • WB, ICC, IF, IHC, Inhib

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Inhibition.

Target

  • Alternative Names
  • MOG; myelin oligodendrocyte glycoprotein; myelin-oligodendrocyte glycoprotein; dystonia 2, torsion (autosomal recessive)

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 8-18C5"

See other products for "MOG"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2266 Hi-Affi™ Recombinant Rabbit Anti-MOG Monoclonal Antibody (DS2266AB) WB, IHC, ICC IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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