Recombinant Mouse anti-Rat NEFL Monoclonal antibody (2E3)
CAT#: MOB-1158CT
Recombinant Mouse monoclonal antibody to Rat NEFL, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, intermediate filaments & midbody.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15021/1910_D3_1_selected.jpg
Normal Tissue
Figure 2 Low expression in tonsil
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_A_6_8_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_B_8_5.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (HEK93, PC-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000277586-NEFL
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Specifications
- Immunogen
- Recombinant full length protein (Rat) expressed in and purified from E. coli.
- Host Species
- Mouse
- Species Reactivity
- Rat
- Clone
- 2E3
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Frozen sections); Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Tissue culture supernatant
- Format
- Liquid
- Concentration
- 1.5 mg/ml
- Buffer
- Preservative: 5mM Sodium Azide Constituents: Tissue Culture Supernatant
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- NEFL; neurofilament, light polypeptide; Nfl; NF-L; neurofilament light polypeptide; 68 kDa neurofilament protein; neurofilament triplet L protein;
- Gene ID
- 83613
- Protein Refseq
- NP_113971
- Function
- identical protein binding; phospholipase binding; protein C-terminus binding; protein domain specific binding; structural constituent of cytoskeleton; structural molecule activity;
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2006z | Mouse Anti-NEFL Recombinant Antibody (clone 32C12) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-0563F | Mouse Anti-NEFL Recombinant Antibody (clone 2F11) | IHC-Fr, IHC-P, IB | Mouse IgG1 |
| VS3-QX806 | Mouse Anti-NEFL Recombinant Antibody (clone 1H3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1150 | Mouse Anti-NEFL Recombinant Antibody (clone 2G10) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS7-0425-WR462 | Mouse Anti-NEFL Recombinant Antibody (clone 2F8B3) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0822MZ | Chicken Anti-NEFL Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1153MZ | Chicken Anti-Neurofilament Light Polyclonal IgY | WB, ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2411 | Hi-Affi™ Recombinant Rabbit Anti-NEFL Monoclonal Antibody (DS2411AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC782 | CytoStream™ Mouse Anti-NEFL Recombinant Antibody (VS13-YC782) | WB, IHC-P, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4777 | Anti-NEFL Immunohistochemistry Kit | IHC | |
| VS-0525-XY4778 | Anti-Mouse NEFL Immunohistochemistry Kit | IHC | |
| VS-0525-XY4780 | Anti-Rat NEFL Immunohistochemistry Kit | IHC |
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