Anti-S. Aureus Recombinant Antibody (TAB-029) (CAT#: TAB-029)

1. Q 1: I'm planning to use this Anti - S. Aureus Recombinant Antibody (Tefibazumab) in an immunofluorescence (IF) experiment to study S. aureus in a cell culture model. Given its high purity, will it bind specifically to the S. aureus ClfA protein without cross - reacting with other proteins in the cell lysate?

   A: This antibody has been confirmed to have high specificity, as it is tested positive against native S. aureus antigen. Its high purity, which is greater than 95% by SDS - PAGE, also reduces the likelihood of cross - reactivity. In an IF experiment, the antibody's specificity towards the ClfA protein of S. aureus should be reliable. However, it's always a good idea to run proper controls, like using a non - specific antibody or pre - absorbing the antibody with the antigen, to ensure that the observed fluorescence is truly due to the binding of Tefibazumab to the ClfA protein and not to other proteins in the cell lysate.

2. Q 2: I want to use this antibody in an ELISA to detect S. aureus in environmental samples. Considering the related disease being staphylococcal infections, will the antibody be able to detect different strains of S. aureus present in these samples?

   A: The Anti - S. Aureus Recombinant Antibody (Tefibazumab) is reactive against S. aureus. While there are many strains of S. aureus, the antibody is targeted against the ClfA protein which is present in S. aureus. However, different strains may have some variability in the ClfA protein structure. In most cases, the antibody should be able to detect different strains as long as the ClfA protein has a sufficient degree of conservation. It's advisable to test the antibody against known different strains of S. aureus in a preliminary experiment to ensure its effectiveness in detecting the specific strains present in your environmental samples.

3. Q 3: Can this antibody be used in a flow cytometry (FC) experiment to analyze the proportion of S. aureus - infected cells in a mixed cell population? If so, what controls should I include?

   A: Yes, this Anti - S. Aureus Recombinant Antibody (Tefibazumab) can be used in FC for such an analysis. As for controls, include an unstained sample to set the baseline fluorescence. A sample stained with an isotype - matched control antibody is also essential. This control helps to account for non - specific binding of the antibody. Given the antibody's high specificity towards S. aureus, it should accurately detect the S. aureus - infected cells in the mixed population. However, optimize the antibody concentration and staining conditions to get the best separation between positive and negative populations.

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