Recombinant Human Anti-S. aureus Hla Antibody (MEDI4893) (CAT#: PABL-396)
Recombinant Human Antibody (MEDI4893) is capable of binding to S. aureus Hla, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-S. aureus Hla mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-S. aureus Hla proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. MEDI4893 recognizes a novel epitope in the so-called "rim" domain of AT and exerts its neutralizing effect through a dual mechanism. In particular, MEDI4893 not only sterically blocks binding of AT to its cell receptor but also prevents it from adopting a lytic heptameric trans-membrane conformation.
Specific Inquiry
Figure 1 Immunofluorescent staining of CCN1 (red) and nuclei (blue) in lung sections from mice immunized (−24 h) with MEDI4893* or c-IgG and then infected with S. aureus for 24 h.
Significance was determined by Mann-Whitney test. Data representative of at least 3 independent experiments (n = 5 mice per group).
Cohen, T. S., Jones-Nelson, O., Hotz, M., Cheng, L., Miller, L. S., Suzich, J., ... & Sellman, B. R. (2016). S. aureus blocks efferocytosis of neutrophils by macrophages through the activity of its virulence factor alpha toxin. Scientific reports, 6, 35466.
Figure 1 Western blot analysis of CCN1 expression in lung homogenates from mice immunized (−24 h) with MEDI4893* or c-IgG and then infected with S. aureus for 24 h.
Significance was determined by Mann-Whitney test. Data representative of at least 3 independent experiments (n = 5 mice per group).
Cohen, T. S., Jones-Nelson, O., Hotz, M., Cheng, L., Miller, L. S., Suzich, J., ... & Sellman, B. R. (2016). S. aureus blocks efferocytosis of neutrophils by macrophages through the activity of its virulence factor alpha toxin. Scientific reports, 6, 35466.
Specifications
- Immunogen
- S. aureus alpha-hemolysin
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native S. aureus Hla
- Species Reactivity
- S. aureus
- Clone
- MEDI4893
- Applications
- WB 1:1000-1:10000
IF Assay-depended
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- Hla; alpha-hemolysin; Alpha-toxin; Staphylococcus aureus alpha toxin
- Gene ID
- 28793
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Human Antibody Products
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-358CL | Anti-Human Hla Recombinant Antibody (TAB-358CL) | FuncS, Neut | Antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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