Recombinant Human Anti-S. aureus Hla Antibody (MEDI4893) (CAT#: PABL-396)
Recombinant Human Antibody (MEDI4893) is capable of binding to S. aureus Hla, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-S. aureus Hla mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-S. aureus Hla proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. MEDI4893 recognizes a novel epitope in the so-called "rim" domain of AT and exerts its neutralizing effect through a dual mechanism. In particular, MEDI4893 not only sterically blocks binding of AT to its cell receptor but also prevents it from adopting a lytic heptameric trans-membrane conformation.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Figure 1 Immunofluorescent staining of CCN1 (red) and nuclei (blue) in lung sections from mice immunized (−24 h) with MEDI4893* or c-IgG and then infected with S. aureus for 24 h.
Significance was determined by Mann-Whitney test. Data representative of at least 3 independent experiments (n = 5 mice per group).
Cohen, T. S., Jones-Nelson, O., Hotz, M., Cheng, L., Miller, L. S., Suzich, J., ... & Sellman, B. R. (2016). S. aureus blocks efferocytosis of neutrophils by macrophages through the activity of its virulence factor alpha toxin. Scientific reports, 6, 35466.
Figure 1 Western blot analysis of CCN1 expression in lung homogenates from mice immunized (−24 h) with MEDI4893* or c-IgG and then infected with S. aureus for 24 h.
Significance was determined by Mann-Whitney test. Data representative of at least 3 independent experiments (n = 5 mice per group).
Cohen, T. S., Jones-Nelson, O., Hotz, M., Cheng, L., Miller, L. S., Suzich, J., ... & Sellman, B. R. (2016). S. aureus blocks efferocytosis of neutrophils by macrophages through the activity of its virulence factor alpha toxin. Scientific reports, 6, 35466.
Specifications
- Immunogen
- S. aureus alpha-hemolysin
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native S. aureus Hla
- Species Reactivity
- S. aureus
- Clone
- MEDI4893
- Applications
- WB 1:1000-1:10000
IF Assay-depended
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- Hla; alpha-hemolysin; Alpha-toxin; Staphylococcus aureus alpha toxin
- Gene ID
- 28793
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone MEDI4893"
See other products for "Hla"
Select a product category from the dropdown menu below to view related products.
Please select product type
Human Antibodies
IgG Antibodies
ScFv Antibodies
Fab Antibodies
ScFv-Fc Chimeras
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-358CL | Anti-Human Hla Recombinant Antibody (TAB-358CL) | FuncS, Neut | Antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABL-396. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: Neut, ELISA, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, IHC, Activ, FuncS, IF, ELISA
Application: ELISA, FC, FuncS
Application: ELISA, Neut, FuncS
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
