Recombinant Human Anti-S. aureus Hla Antibody (MEDI4893)
CAT#: PABL-396
Recombinant Human Antibody (MEDI4893) is capable of binding to S. aureus Hla, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-S. aureus Hla mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-S. aureus Hla proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. MEDI4893 recognizes a novel epitope in the so-called "rim" domain of AT and exerts its neutralizing effect through a dual mechanism. In particular, MEDI4893 not only sterically blocks binding of AT to its cell receptor but also prevents it from adopting a lytic heptameric trans-membrane conformation.
Published Data
Figure 1 Immunofluorescent staining of CCN1 (red) and nuclei (blue) in lung sections from mice immunized (−24 h) with MEDI4893* or c-IgG and then infected with S. aureus for 24 h.
Significance was determined by Mann-Whitney test. Data representative of at least 3 independent experiments (n = 5 mice per group).
Cohen, T. S., Jones-Nelson, O., Hotz, M., Cheng, L., Miller, L. S., Suzich, J., ... & Sellman, B. R. (2016). S. aureus blocks efferocytosis of neutrophils by macrophages through the activity of its virulence factor alpha toxin. Scientific reports, 6, 35466.
Figure 1 Western blot analysis of CCN1 expression in lung homogenates from mice immunized (−24 h) with MEDI4893* or c-IgG and then infected with S. aureus for 24 h.
Significance was determined by Mann-Whitney test. Data representative of at least 3 independent experiments (n = 5 mice per group).
Cohen, T. S., Jones-Nelson, O., Hotz, M., Cheng, L., Miller, L. S., Suzich, J., ... & Sellman, B. R. (2016). S. aureus blocks efferocytosis of neutrophils by macrophages through the activity of its virulence factor alpha toxin. Scientific reports, 6, 35466.
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Specifications
- Immunogen
- S. aureus alpha-hemolysin
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native S. aureus Hla
- Species Reactivity
- S. aureus
- Clone
- MEDI4893
- Applications
- WB 1:1000-1:10000
IF Assay-depended
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- Hla; alpha-hemolysin; Alpha-toxin; Staphylococcus aureus alpha toxin
- Gene ID
- 28793
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-358CL | Anti-Human Hla Recombinant Antibody (TAB-358CL) | FuncS, Neut | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-332 | Recombinant Human Anti-S.Aureus a-hemolysin Antibody | WB, ELISA, IF, FuncS | IgG |
| PABL-696 | Recombinant Human Anti-S. aureus a-hemolysin Antibody | WB, IHC, IF, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-332 | Recombinant Human Anti-S.Aureus a-hemolysin Antibody scFv Fragment | WB, ELISA, IF, FuncS | scFv |
| PSBL-395 | Recombinant Human Anti-S. aureus Hla Antibody scFv Fragment (MEDI4893) | BL | scFv |
| PSBL-689 | Recombinant Human Anti-S. aureus a-hemolysin Antibody scFv Fragment | WB, IHC, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-332 | Recombinant Human Anti-S.Aureus a-hemolysin Antibody Fab Fragment | WB, ELISA, IF, FuncS | Fab |
| PFBL-395 | Recombinant Human Anti-S. aureus Hla Antibody Fab Fragment (MEDI4893) | BL | Fab |
| PFBL-689 | Recombinant Human Anti-S. aureus a-hemolysin Antibody Fab Fragment | WB, IHC, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY151 | Human Anti-hla (clone MEDI4893) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
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