Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (DS3160AB)
CAT#: MOR-3160
Recombinant Rabbit anti-S100B monoclonal antibody for EIA, IP, RIA, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
Normal Tissue
Figure 6 Soft tissue
Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (SK-MEL-30, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
❮
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Specifications
- Immunogen
- Human S100 beta protein purified from Human plasma.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS3160AB
- Applications
- Used for immunoassay techniques such as: Enzyme Immunoassay; Immunoprecipitation; Radioimmunoassay; Western Blot
- Conjugate
- Biotin
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "S100B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2226-sdAb | Recombinant Anti-Human S100B VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1032 | Recombinant Mouse Anti-Human S100B Antibody Fab Fragment | ELISA | Fab |
| HPAB-1326LY-F(E) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); Fab Fragment | Neut, IHC, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1445MZ | Recombinant Mouse Anti-Human S100B Antibody (clone 7H2) | WB | Mouse antibody |
| ZG-0614F | Mouse Anti-S100B Recombinant Antibody (clone SH-B1) | IHC-P | Mouse IgG1 |
| VS-0223-WK60 | Rabbit Anti-S100b Recombinant Antibody (VS-0223-WK60) | ICC, IHC, IHC-P | Rabbit IgG |
| VS-0923-FY129 | Recombinant Mouse Anti-S100B Antibody (VS-0923-FY129) | ELISA, WB | Mouse IgG1 |
| VS3-WK1666 | Recombinant Mouse Anti-S100B Capture Antibody (clone 13F5) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4729 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (TH243DS) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1326LY-S(P) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); scfv Fragment | Neut, IHC, ELISA | Mouse scfv |
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