Anti-SARS-CoV S Recombinant Antibody (CR3014) (CAT#: MRO-1213LC)

This product is a recombinant human anti-SARS-CoV monoclonal antibody. CR3014 isolated from a phage display library specifically binds to spike receptor binding domain associated with SARS-CoV and can be potentially used in the diagnosis and treatment studies of SARS-CoV infection.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA
Neut

Figure 1 Neutralization of Wild-Type SARS-CoV and a CR3014- Neutralization Escape Variant (E6) with mAbs CR3014 and CR3022 Individually and in Combination.

Figure 1 Neutralization of Wild-Type SARS-CoV and a CR3014- Neutralization Escape Variant (E6) with mAbs CR3014 and CR3022 Individually and in Combination.

Neutralization of 100 TCID₅₀ of each virus was performed in octuplicate.

Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants

ELISA

Figure 2 Binding of mAbs CR3014 and CR3022 to Recombinant WildType and P462L-Substituted S318–510 Fragments Analyzed by ELISA Bars represent the means 6 standard deviation.

Figure 2 Binding of mAbs CR3014 and CR3022 to Recombinant WildType and P462L-Substituted S318–510 Fragments Analyzed by ELISA Bars represent the means 6 standard deviation.

Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants

ELISA

Figure 3 Competition ELISA on Immobilized S318–510.

Figure 3 Competition ELISA on Immobilized S318–510.

Binding of biotinylated CR3022 was analyzed in the presence of competitor CR3014 (open circles), CR3022 (closed circles), and control mAb (open squares). Binding is expressed as percentage of binding without competitor.

Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants

IF

Figure 4 Binding of the IgGs to SARS-CoV-infected Vero cells.

Figure 4 Binding of the IgGs to SARS-CoV-infected Vero cells.

Immunofluorescent staining with IgG CR3014, or negative control IgG (D) is shown.

Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus

Inhib

Figure 5 Inhibition of the interaction of S565 with Vero cells by human IgG measured by fluorescence-activated cell sorting.

Figure 5 Inhibition of the interaction of S565 with Vero cells by human IgG measured by fluorescence-activated cell sorting.

Vero cells were incubated with S-protein-derived fragment S565 in the absence (thin line) or presence (solid line) of IgG CR3014. The dotted line represents the negative control (irrelevant myc-tagged protein).

Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus

Neut

Figure 6 SARS-CoV neutralizing activity of scFv and IgGs. Neutralization is shown as percentage.

Figure 6 SARS-CoV neutralizing activity of scFv and IgGs. Neutralization is shown as percentage.

Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus


Specifications

  • Host Species
  • Human
  • Derivation
  • Phage display library
  • Type
  • Human antibody
  • Specificity
  • SARS-CoV
  • Clone
  • CR3014
  • Applications
  • ELISA, Neut, IF
  • Related Disease
  • SARS-CoV infection

Applications

  • Application Notes
  • The SARS-CoV S antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization, Immunofluorescence.
    Neut: 60 μg/ml

Target

  • Alternative Names
  • SARS coronavirus; SARS-CoV; SARS coronavirus spike protein; SARS-CoV S

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone CR3014"

See other products for "SARS-CoV S"

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-1139CQ Recombinant Human Anti-SARS-CoV S Antibody (m396) ELISA IgG1

Recombinant Antibody

Single-domain Antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for MRO-1213LC. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare