Human Anti-SHH Recombinant Antibody (clone 5E1); Fab Fragment
CAT#: PFBL-696
Recombinant Chimeric (Human/Mouse) Antibody (5E1) Fab Fragment is specific to SHH. This Fab fragment is a Hh pathway inhibitor that has been extensively used to elucidate vertebrate Hh biology due to its ability to block binding of the three mammalian Hh homologs to the receptor.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (EFO-21, GAMG, OE19, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164690-SHH/subcellular
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Specifications
- Immunogen
- Human sonic hedgehog
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Fab
- Specificity
- Human SHH
- Species Reactivity
- Human
- Clone
- 5E1
- Applications
- Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Potent Pathway Inhibitor
The 5E1 Fab fragment is the gold standard for blocking Sonic Hedgehog signaling, and this product did not disappoint. We used it to inhibit the Hh pathway in our embryonic development models, and it effectively blocked ligand binding as expected.
Essential for SHH Research
This recombinant Fab fragment against Human SHH is a crucial reagent for our lab. It allows us to specifically neutralize the signaling activity of the N-terminal product. The purity was high, and it has been instrumental in elucidating vertebrate hedgehog biology.
Q&As
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Does this Fab fragment inhibit the Hedgehog pathway?
A: Yes, this recombinant chimeric (Human/Mouse) Fab fragment (clone 5E1) acts as a Hh pathway inhibitor. It has the ability to block the binding of mammalian Hedgehog homologs (including SHH) to their receptor, making it useful for neutralization studies.
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What is the specific target of this antibody fragment?
A: This antibody fragment is specific to Human SHH (Sonic Hedgehog). It recognizes the SHH protein, which is a key inductive signal in embryonic patterning. It is provided as a Fab fragment.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Signaling Pathways
JAK-STAT Signaling Pathway
Related Diseases
Pancreatic Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 5E1"
- CAT
- Product Name
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|---|---|---|---|
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|---|---|---|---|
| MOB-902-F(E) | Recombinant Anti-human SHH Antibody Fab Fragment | IF, FC, FuncS | Fab |
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| CAT | Product Name | Application | Type |
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| MHH-902 | Recombinant Human Anti-human SHH Antibody | WB, IHC, FuncS | IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0712-CN-F(E) | Human Anti-SHH Recombinant Antibody (clone 3H8OP); Fab Fragment | ELISA, FC | Human Fab |
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