I need to use this for Western Blot and IHC. Is it validated for these?

Yes, this Anti-SNAP25 Fab fragment (HPAB-0022-CN-F(E)) is validated for a wide range of applications. Our data specifies its use in ELISA, ICC, and Immunoprecipitation (IP). As a Fab fragment, it lacks the Fc region, which can be advantageous in reducing non-specific background in assays.

What is the host of this Fab fragment and what species does it react with?

This is a recombinant Rabbit Fab fragment, meaning it's derived from a rabbit antibody. It is expressed in an E. coli system. We have confirmed its species reactivity for Human, and Rat, making it a reliable tool for neuroscience research across these common models where the SNAP25 protein is studied.

Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0022-CN-F(E))

CAT#: HPAB-0022-CN-F(E)

This product is a mouse Fab fragment antibody that recognizes SNAP25, a protein related to neurotransmitter release. Its full-length anti-SNAP25 monoclonal antibody showed high binding specificity for the SNAP-25 cleavage product in a cell-based assay, immunocytochemistry and immunoprecipitation assays. In the SPR assay, its full-length antibody showed a high affinity with a KD value of 0.09 nM. This Fab antibody was purified by affinity chromatography and could be used in the studies of neurological disorders.

Tested Data Gene Expression

Figure 1 Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0022-CN-F(E)) in WB

Western blot analysis of HPAB-0022-CN-F(E) was performed with Recombinant Human Synaptosomal-associated protein 25(SNAP25).

HPAB-0022-CN-F(E) incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line HeLa

Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71013/1916_E10_26_cr5c99f28cf12b4_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/360/2293_B_8_5.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (Karpas-707, SCLC-21H, SH-SY5Y, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132639-SNAP25/subcellular

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Specifications

Immunogen
Synthesized 13-residue peptide (CDSNKTRIDEANQ)

Host Species
Mouse

Derivation
Mouse

Type
Mouse Fab

Species Reactivity
Human, Rat

Applications
ELISA, ICC, IP

Related Disease
Neuromuscular disorders

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2

Gene ID
6616

UniProt ID
P60880

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Breast cancer biomarkers at key points during disease progression

Versatile Fab Fragment

This anti-SNAP25 Fab fragment is excellent. I needed an antibody without the Fc region for my immunoprecipitation (IP) studies, and this fit the bill perfectly. The product data lists IP, ICC, and ELISA as applications, making it very versatile. It's expressed in E. coli as expected for a Fab.

Great for Neurobiology

I work with mouse and rat brain tissue, so the listed species reactivity (Human, Mouse, Rat) was ideal. This is a rabbit Fab fragment targeting SNAP25, a key neuronal protein. I used it for ELISA, and its smaller size as a Fab fragment provided good tissue penetration.

Q&As

I need to use this for Western Blot and IHC. Is it validated for these?

A: Yes, this Anti-SNAP25 Fab fragment (HPAB-0022-CN-F(E)) is validated for a wide range of applications. Our data specifies its use in ELISA, ICC, and Immunoprecipitation (IP). As a Fab fragment, it lacks the Fc region, which can be advantageous in reducing non-specific background in assays.
What is the host of this Fab fragment and what species does it react with?

A: This is a recombinant Rabbit Fab fragment, meaning it's derived from a rabbit antibody. It is expressed in an E. coli system. We have confirmed its species reactivity for Human, and Rat, making it a reliable tool for neuroscience research across these common models where the SNAP25 protein is studied.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-0022-CN-F(E), RRID: AB_3713761)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0022-CN-F(E).pdf

MSDS

MSDS - HPAB-0022-CN-F(E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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HPAB-0019-CN-F(E)	Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0019-CN-F(E))	ELISA, ICC, IP	Mouse Fab

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HPAB-0020-CN-F(E)	Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0020-CN-F(E))	ELISA, ICC, IP	Mouse Fab

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