Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0022-CN-F(E))
CAT#: HPAB-0022-CN-F(E)
This product is a mouse Fab fragment antibody that recognizes SNAP25, a protein related to neurotransmitter release. Its full-length anti-SNAP25 monoclonal antibody showed high binding specificity for the SNAP-25 cleavage product in a cell-based assay, immunocytochemistry and immunoprecipitation assays. In the SPR assay, its full-length antibody showed a high affinity with a KD value of 0.09 nM. This Fab antibody was purified by affinity chromatography and could be used in the studies of neurological disorders.
Tested Data
Gene Expression
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WB
Figure 1 Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0022-CN-F(E)) in WB
Western blot analysis of HPAB-0022-CN-F(E) was performed with Recombinant Human Synaptosomal-associated protein 25(SNAP25).
HPAB-0022-CN-F(E) incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71013/1916_E10_26_cr5c99f28cf12b4_selected.jpg
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Specifications
- Immunogen
- Synthesized 13-residue peptide (CDSNKTRIDEANQ)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse Fab
- Species Reactivity
- Human, Rat
- Applications
- ELISA, ICC, IP
- Related Disease
- Neuromuscular disorders
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0019-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0019-CN) | ELISA, ICC, IP | Mouse IgG2, κ |
| HPAB-0021-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0021-CN) | ELISA, ICC, IP | Mouse IgG3, κ |
| HPAB-0023-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0023-CN) | ELISA, ICC, IHC | Mouse IgG2a, κ |
| HPAB-0024-CN | Human Anti-SNAP25 Recombinant Antibody (clone h3C1A5) | ELISA, ICC, IP, IF | Humanized IgG1 |
| VS-0223-WK62 | Rabbit Anti-SNAP25 Recombinant Antibody (VS-0223-WK62) | WB, IP, ICC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0020-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0020-CN) | ELISA, ICC, IP | Mouse IgG, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0019-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0019-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0020-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0020-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0021-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0021-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0023-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0023-CN-F(E)) | ELISA, ICC, IHC | Mouse Fab |
| HPAB-0024-CN-F(E) | Human Anti-SNAP25 Recombinant Antibody (clone h3C1A5); Fab Fragment | ELISA, ICC, IP, IF | Human Fab |
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| CAT | Product Name | Application | Type |
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| VS-1024-XY488 | Mouse Anti-NHP SNAP25 Recombinant Antibody (clone S-7B8) | IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0325-XY2128 | Anti-SNAP25 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6768 | Anti-Mouse SNAP25 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6767 | Anti-Human SNAP25 Immunohistochemistry Kit | IHC |
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