Human Anti-SNAP25 Recombinant Antibody (clone h3C1A5) (CAT#: HPAB-0024-CN)
An humanized antibody derived from the murine antibody, m3C1A5. The human mAb directed against BoNT/A-cleaved SNAP25 was very specific for SNAP25197.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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DB
Figure 3 Anti-SNAP25 Antibody (HPAB-0024-CN) in Dot Blot
Dot Blot analysis of HPAB-0024-CN was performed by coating with Recombinant Human SNAP-25 Protein (his Tag).
HPAB-0024-CN incubation concentration: 1 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 1 Anti-SNAP25 Antibody (HPAB-0024-CN) in ELISA
ELISA analysis of HPAB-0024-CN was performed by coating with Recombinant Human SNAP-25 Protein (his Tag). Then blocked with BSA and incubated with Anti-Human SNAP-25 Antibody (HPAB-0024-CN). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-SNAP25 Antibody (HPAB-0024-CN) in WB
Western blot analysis of HPAB-0024-CN was performed by loading Recombinant Human SNAP-25 Protein (his Tag) onto a 12% Tris-HCl polyacrylamide gel. Membranes were probed with HPAB-0024-CN and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Humanized IgG1
- Species Reactivity
- Human, Rat
- Clone
- h3C1A5
- Applications
- ELISA, ICC, IP, IF
- Related Disease
- Neuromuscular disorders
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
- Gene ID
- 6616
- UniProt ID
- P60880
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-912 | Recombinant Human Anti-SNAP25 Antibody | WB, FuncS | IgG |
| MOB-1896CT | Recombinant Mouse anti-Human SNAP25 Monoclonal antibody (TQ23) | ELISA, IHC-P, WB | |
| FAMAB-1551CQ | Mouse Anti-SNAP-25 Recombinant Antibody (clone SP12) | WB, ELISA, IF, IHC | Mouse IgG1 |
| VS3-FY1365 | Recombinant Rabbit Anti-SNAP25 Antibody (clone R07-1D7) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY1882 | Rabbit Anti-SNAP25 Recombinant Antibody (clone R01-1C6) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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