An humanized antibody derived from the murine antibody, m3C1A5. The human mAb directed against BoNT/A-cleaved SNAP25 was very specific for SNAP25197.
Figure 3 Anti-SNAP25 Antibody (HPAB-0024-CN) in Dot Blot
Dot Blot analysis of HPAB-0024-CN was performed by coating with Recombinant Human SNAP-25 Protein (his Tag).
HPAB-0024-CN incubation concentration: 1 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 1 Anti-SNAP25 Antibody (HPAB-0024-CN) in ELISA
ELISA analysis of HPAB-0024-CN was performed by coating with Recombinant Human SNAP-25 Protein (his Tag). Then blocked with BSA and incubated with Anti-Human SNAP-25 Antibody (HPAB-0024-CN). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-SNAP25 Antibody (HPAB-0024-CN) in WB
Western blot analysis of HPAB-0024-CN was performed by loading Recombinant Human SNAP-25 Protein (his Tag) onto a 12% Tris-HCl polyacrylamide gel. Membranes were probed with HPAB-0024-CN and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-912 | Recombinant Human Anti-SNAP25 Antibody | WB, FuncS | IgG |
MOB-0211MZ | Recombinant Mouse Anti-SNAP-25 (amino acid 183-197) cleaved Antibody (clone C318M) | ELISA, IHC | Mouse antibody |
HPAB-0019-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0019-CN) | ELISA, ICC, IP | Mouse IgG2, κ |
HPAB-0020-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0020-CN) | ELISA, ICC, IP | Mouse IgM, κ |
HPAB-0021-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0021-CN) | ELISA, ICC, IP | Mouse IgG3, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-912-F(E) | Recombinant Human Anti-SNAP25 Antibody Fab Fragment | WB, ELISA, IHC, FuncS | Fab |
HPAB-0019-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0019-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
HPAB-0020-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0020-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
HPAB-0021-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0021-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
HPAB-0022-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0022-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-912-S(P) | Recombinant Human Anti-SNAP25 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
HPAB-0019-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0019-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
HPAB-0020-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0020-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
HPAB-0021-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0021-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
HPAB-0022-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0022-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-3351 | Hi-Affi™ Recombinant Rabbit Anti-SNAP25 Monoclonal Antibody (DS3351AB) | WB, IP, ICC/IF | IgG |
There are currently no Customer reviews or questions for HPAB-0024-CN. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.