Anti-SNCA Intrabody [Tat] (IAB-B031(T))
Recombinant anti-SNCA intrabody in fusion with HIV Tat works within cells to bind human monomeric wild-type SNCA, expressed in E. coli cells. The intrabody stabilizes detergent-soluble monomeric syn and inhibits the formation of detergent-insoluble high-molecular-weight syn species. In addition, the intrabody ameliorates the decreased cell adhesion that characterizes the syn-overexpressing cells. Anti-SNCA intrabodies may represent novel molecular therapeutics for synucleinopathies, with implications for other neurodegenerative disorders caused by misfolded accumulated proteins.
Specifications
- Immunogen
- human synuclein, alpha (non A4 component of amyloid precursor)
- Host
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Products can be used for: Enzyme-linked Immunosorbent Assay; Immunofluorescence; Neutralization; Functional studies
Target
- Alternative Names
- SNCA; synuclein, alpha (non A4 component of amyloid precursor); PD1; NACP; PARK1; PARK4; alpha-synuclein; synuclein alpha-140
- Entrez Gene ID
- 6622
- UniProt ID
- P37840
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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