Mouse Anti-SNCA Recombinant Antibody (TAB-0750CLV) (CAT#: TAB-0750CLV)
This product is a recombinant mouse antibody that recognizes α-synuclein, with a high affinity for aggregated forms of α-synuclein. The binding affinities of this antibody were determined by a variety of methods including isothermal calorimetry, ELISA, and surface plasmon resonance-based approaches. The IC₅₀ value for fibrils is 0.5 nM while for monomers is 3.6 μM. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Specific Inquiry
ELISA
Figure 1 Anti-Human SNCA Antibody (TAB-0750CLV) in ELISA
ELISA analysis of TAB-0750CLV was performed by coating with Recombinant human alpha-Synuclein Protein (12093-HNAE). Then blocked with BSA and incubated with Anti-Human SNCA Antibody (Syn-O4) (TAB-0750CLV) at a starting concentration of 1 ng/mL. The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human SNCA Antibody (TAB-0750CLV) in WB
Western blot analysis was performed by loading Recombinant human alpha-Synuclein Protein (12093-HNAE) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human SNCA Antibody (Syn-O4) (TAB-0750CLV) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Non-Reducing Antigen (2.5ug)
Lane 2: Reducing Antigen (2.5ug)
SDS-PAGE
Figure 3 Anti-Human SNCA Antibody (TAB-0750CLV) in SDS-PAGE
SDS-PAGE analysis of TAB-0750CLV in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 4 Anti-Human SNCA Antibody (TAB-0750CLV) in HPLC
The purity of TAB-0750CLV was greater than 95% as determined by HPLC.
Specifications
- Immunogen
- α-synuclein oligomers
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SNCA
- Species Reactivity
- Human
- Applications
- ELISA
- Related Disease
- Parkinson's disease, dementia with Lewy bodies, multiple system atrophy
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SNCA; synuclein, alpha; PD1; NACP; PARK1; PARK4; Alpha-synuclei
- Gene ID
- 6622
- UniProt ID
- P37840
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SNCA"
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B031(A) | Recombinant Anti-human SNCA Intrabody [(D-Arg)9] | FC, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B031(G) | Recombinant Anti-human SNCA Intrabody [+36 GFP] | CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B031(T) | Recombinant Anti-human SNCA Intrabody [Tat] | ELISA, IF, Neut, FuncS | scFv-Tat |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-086 | Recombinant Anti-SNCA VHH Single Domain Antibody (NbSyn2) | WB, FuncS | Llama VHH |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0740CLV | Rabbit Anti-SNCA Recombinant Antibody (TAB-0740CLV) | ELISA | Chimeric antibody (mouse/rabbit) |
| TAB-0740CLV-F(E) | Rabbit Anti-SNCA Recombinant Antibody; Fab Fragment (TAB-0740CLV-F(E)) | ELISA | Chimeric antibody (mouse/rabbit) |
| TAB-0740CLV-S(P) | Rabbit Anti-SNCA Recombinant Antibody; scFv Fragment (TAB-0740CLV-S(P)) | ELISA | scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0748CLV-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (TAB-0748CLV-S(P)) | ELISA | Mouse scFv |
| TAB-0749CLV-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (TAB-0749CLV-S(P)) | ELISA | Mouse scFv |
| TAB-0750CLV-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (TAB-0750CLV-S(P)) | ELISA | Mouse scFv |
| TAB-0751CLV-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (TAB-0751CLV-S(P)) | ELISA | Mouse scFv |
| TAB-0752CLV-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (TAB-0752CLV-S(P)) | ELISA | Mouse scFv |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1977CQ | Recombinant Human Anti-SNCA Antibody (NAC32) | IHC, WB, BL | IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0154 | Hi-Affi™ Recombinant Rabbit Anti-alpha + beta Synuclein Monoclonal Antibody (DS154AB) | FC, WB, ICC/IF | IgG |
| MOR-3354 | Hi-Affi™ Recombinant Rabbit Anti-SNCA Monoclonal Antibody (DS3354AB) | IHC, ICC, FC, IP | IgG |
| MOR-4530 | Hi-Affi™ Recombinant Rabbit Anti-SNCA Monoclonal Antibody (TH38DS) | WB, IF, ICC, IHC-P, FC | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-090LC | Recombinant Human Anti-SNCA Recombinant Antibody (aSynA) | ELISA, FuncS, WB | Human IgG1, κ |
| MRO-2298-CN | Recombinant Rabbit Anti-SNCA (phosphorylated Ser129) Monoclonal Antibody (JB22-44) | WB, IF, IHC | Rabbit IgG |
| VS3-XY1446 | Mouse Anti-SNCA Recombinant Antibody (clone 2D4) | ELISA | |
| VS3-XY1447 | Mouse Anti-SNCA Recombinant Antibody (clone 2H5) | ELISA | |
| VS3-XY1448 | Mouse Anti-SNCA Recombinant Antibody (clone 2G5) | WB, IP, IF | Mouse IgG1 |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-005CN | Mouse Anti-SNCA Recombinant Antibody (NS-005CN) | WB, ELISA, FC | Mouse IgG2a |
| NS-088CN | Mouse Anti-SNCA Recombinant Antibody (NS-088CN) | WB, ELISA, IHC | Mouse IgG2a |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-005CN-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (NS-005CN-S(P)) | WB, ELISA, FC | Mouse scFv |
| NS-088CN-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (NS-088CN-S(P)) | WB, ELISA, IHC | Mouse scFv |
| FAMAB-0260-CN-S(P) | Human Anti-SNCA Recombinant Antibody (clone D10); scFv Fragment | ELISA | Human scFv |
| HPAB-653-FY-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (HPAB-653-FY-S(P)) | WB, IP, ELISA | Mouse scFv |
| HPAB-654-FY-S(P) | Mouse Anti-SNCA Recombinant Antibody; scFv Fragment (HPAB-654-FY-S(P)) | ELISA | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0260-CN-F(E) | Human Anti-SNCA Recombinant Antibody (clone D10); Fab Fragment | ELISA | Human Fab |
| FAMAB-0261-CN-F(E) | Human Anti-SNCA Recombinant Antibody (clone 6E); Fab Fragment | ELISA | Human Fab |
| HPAB-0023-FY-F(E) | Mouse Anti-SNCA Recombinant Antibody; Fab Fragment (HPAB-0023-FY-F(E)) | ELISA | Mouse Fab |
| HPAB-653-FY-F(E) | Mouse Anti-SNCA Recombinant Antibody; Fab Fragment (HPAB-653-FY-F(E)) | WB, IP, ELISA | Mouse Fab |
| HPAB-654-FY-F(E) | Mouse Anti-SNCA Recombinant Antibody; Fab Fragment (HPAB-654-FY-F(E)) | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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