Mouse Anti-SOST Recombinant Antibody (MOB-012LC)

Customer Review

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Exceptional quality and reliability

Exceptional quality and reliability! We routinely use this in our laboratory. The consistency is remarkable, and the clarity of results is unparalleled.

Great for ELISA

Great antibody for scientific research. It fulfilled all our experimental needs, especially for ELISA. One star deducted for the high cost compared to other similar products.

Consistent and clear results in ELISA

In our bone metabolism research, the product performed exceptionally well. We've used it frequently in ELISA, and the findings have been consistent and clear every time.

J**n

Excellent for WB

The anti-SOST antibody (MOB-012LC) works well in Western blot. It bound to human Sclerostin protein at 1/2000 dilution.

Q&As

1. What are the advantages of using a recombinant antibody like MOB-012LC over a polyclonal antibody?

   A: Recombinant antibodies, such as MOB-012LC, are highly reliable for research purposes due to consistent performance across different batches, reduced variability from lot to lot, and exceptional specificity.

2. What is the concentration of the stock solution for this antibody?

   A: Typically supplied at a 1 mg/mL concentration, the stock solution's precise details can be confirmed by consulting the datasheet.

3. Can this antibody be used for flow cytometry?

   A: This product has not been validated for flow cytometry. For studies employing this technique, we recommend using antibodies specifically certified for flow cytometry applications.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. De Maré, Annelies, et al. "Sclerostin protects against vascular calcification development in mice." Journal of Bone and Mineral Research 37.4 (2020): 687-699. https://doi.org/10.1002/jbmr.4503
   This research investigates the role of sclerostin, a negative regulator of Wnt/β-catenin signaling, in the development of vascular calcification. Using both Sost knockout mice (Sost-/-) and anti-sclerostin antibody treatment in two different mouse models of vascular calcification, the study demonstrates that the absence or inhibition of sclerostin promotes vascular calcification. In the adenine-induced chronic kidney disease model, Sost-/- mice developed more extensive cardiac vessel calcifications compared to wild-type mice. Similarly, in the warfarin-induced calcification model (without kidney disease), mice treated with anti-sclerostin antibody showed significantly increased aortic and renal vessel calcification compared to vehicle-treated controls, accompanied by upregulation of Wnt target genes in the vascular tissue.
   Creative Biolabs provided the recombinant anti-SOST therapeutic antibody (MOB-012LC) used in the experimental treatments. This antibody was administered subcutaneously at a dose of 25 mg/kg twice weekly to investigate the effects of sclerostin inhibition on vascular calcification development. The antibody effectively blocked sclerostin function as evidenced by increased bone formation parameters, providing a critical tool that helped establish sclerostin's protective role in vascular calcification. This finding has important implications for therapeutic applications of anti-sclerostin antibodies, suggesting potential cardiovascular risks that should be monitored in patients receiving such treatments, particularly those with chronic kidney disease.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MOB-012LC, RRID: AB_3713908)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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