Hi-Affi™ Recombinant Rabbit Anti-STAT6 Monoclonal Antibody (TH227DS)
CAT#: MOR-4713
Recombinant Rabbit anti-STAT6 monoclonal antibody for WB, IF, ICC, IHC-P, FCM, ChIP, ELISA, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1861/27_A4_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1861/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1861/7497_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1861/7497_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1861/7497_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1861/7497_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1861/7497_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166888-STAT6/subcellular
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Specifications
- Immunogen
- APeptide corresponding to amino acids 636-645 of P42226.
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human, Mouse
- Clone
- TH227DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Immunohistochemistry (Paraffin); Flow Cytometry; Chromatin immunoprecipitation; Enzyme-linked Immunosorbent Assay
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- ChIP: 3 µg; ELISA: 1-5 µg/ml; FC: 2-4 µg x 10^6 cells; ICC: 2-4 µg; IF: 2-4 µg; IHC-P: 1:10-1:100; WB: 0.5-1 µg/ml
Target
- Alternative Names
- Signal Transducer And Activator Of Transcription 6; Signal Transducer And Activator Of Transcription 6, Interleukin-4 Induced 2; Transcription Factor IL-4 STAT; STAT, Interleukin4-Induced; EC 2.4.1.227; EC 2.7.7.6; IL-4-STAT; IL-4 Stat; D12S1644; STAT6B; STAT6C
- Gene ID
- 6778
- UniProt ID
- P42226
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Related Diseases
Endometrial Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Throid Cancer
Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "STAT6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1957CT | Recombinant Mouse anti-Human STAT6 Monoclonal antibody (3H7) | WB | |
| VS-0923-FY192 | Recombinant Mouse Anti-STAT6 Antibody (VS-0923-FY192) | ELISA, WB | Mouse IgG2a |
| VS-0923-FY193 | Recombinant Mouse Anti-STAT6 Antibody (VS-0923-FY193) | ELISA, WB | Mouse IgG1 |
| VS7-0425-WR929 | Mouse Anti-STAT6 Monoclonal Antibody (clone 7D3) | WB, ICC | Mouse IgG1 |
| VS7-0425-WR930 | Mouse Anti-STAT6 Monoclonal Antibody (VS7-0425-WR930) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0567MZ | Chicken Anti-STAT6 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3430 | Hi-Affi™ Recombinant Rabbit Anti-STAT6 Monoclonal Antibody (DS3430AB) | FC, ICC, IP, WB | IgG |
| MOR-4745 | Hi-Affi™ Recombinant Rabbit Anti-STAT6 Monoclonal Antibody (TH259DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC633 | AbPlus™ Anti-STAT6 Magnetic Beads (VS-0724-YC633) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2198 | Anti-STAT6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6961 | Anti-Mouse STAT6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6960 | Anti-Human STAT6 Immunohistochemistry Kit | IHC |
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