Human Anti-Siglec15 Recombinant Antibody (TAB-447CT) (CAT#: TAB-447CT)
This product is a recombinant human antibody that recognizes Siglec-15. human Siglec-15 was used as a immunogen. The antibody was purified by affinity chromatography. It can be used for WB, ELISA, FC and other applications.
Specific Inquiry
SDS-PAGE
Figure 1 Human Anti-SIGLEC15 Recombinant Antibody (TAB-447CT) in SDS-PAGE
SDS-PAGE analysis of TAB-447CT in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
WB
Figure 2 Human Anti-SIGLEC15 Recombinant Antibody (TAB-447CT) in WB
Western blot analysis of TAB-447CT was performed by loading Human Siglec-15 / CD33L3 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-447CT and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)
DB
Figure 3 Human Anti-SIGLEC15 Recombinant Antibody (TAB-447CT) in DB
Dot Blot analysis of TAB-447CT was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag).
TAB-447CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 4 Human Anti-SIGLEC15 Recombinant Antibody (TAB-447CT) in ELISA
ELISA analysis of TAB-447CT was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag). Then blocked with BSA, and incubated with TAB-447CT. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
ELISA
Figure 5 Anti-Human Siglec-15 Recombinant Antibody (TAB-447CT) in ELISA
ELISA analysis of TAB-447CT was performed by coating with human Siglec-15 protein (His tag). Then blocked with BSA and incubated with TAB-447CT. The HRP conjugated Anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Human Siglec-15
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Human
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Western Blot; Enzyme-linked Immunosorbent Assay; Flow Cytometry
- Related Disease
- Acute myeloid leukaemia
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SIGLEC15; sialic acid binding Ig-like lectin 15; CD33L3; HsT1361; SIGLEC-15; sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; CD33 molecule-like 3
- Gene ID
- 284266
- UniProt ID
- Q6ZMC9
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-904-F(E) | Recombinant Human Anti-human SIGLEC15 Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| HPAB-0039-WJ-F(E) | Human Anti-SIGLEC15 Recombinant Antibody; Fab Fragment (HPAB-0039-WJ-F(E)) | ELISA, WB | Human Fab |
| HPAB-0040-WJ-F(E) | Human Anti-SIGLEC15 Recombinant Antibody; Fab Fragment (HPAB-0040-WJ-F(E)) | ELISA, WB | Humanized Fab |
| HPAB-675-FY-F(E) | Human Anti-SIGLEC15 Recombinant Antibody; Fab Fragment (HPAB-675-FY-F(E)) | ELISA | Human Fab |
| HPAB-AP449-YC-F(E) | Rat Anti-SIGLEC15 Recombinant Antibody (clone 24A3); Fab Fragment | Inhib, FuncS | Rat Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-568CL | Human Anti-SIGLEC15 Recombinant Antibody (TAB-568CL) | ELISA | Human IgG2 |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-441CT | Chimeric Anti-Siglec15 Recombinant Antibody (TAB-441CT) | ELISA, FC, BL | Chimeric antibody (mouse/human) |
| TAB-442CT | Chimeric Anti-Siglec15 Recombinant Antibody (TAB-442CT) | ELISA, FC, BL | Chimeric antibody (mouse/human) |
| TAB-443CT | Chimeric Anti-Siglec15 Recombinant Antibody (TAB-443CT) | ELISA, FC, BL | Chimeric antibody (mouse/human) |
| TAB-444CT | Chimeric Anti-Siglec15 Recombinant Antibody (TAB-444CT) | ELISA, FC, BL | Chimeric antibody (mouse/human) |
| TAB-445CT | Chimeric Anti-Siglec15 Recombinant Antibody (TAB-445CT) | ELISA, FC, BL | Chimeric antibody (mouse/human) |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-448CT | Human Anti-Siglec15 Recombinant Antibody (TAB-448CT) | WB, ELISA, FC | Human IgG |
| TAB-447CT-S(P) | Human Anti-Siglec15 Recombinant Antibody; scFv Fragment (TAB-447CT-S(P)) | WB, ELISA, FC | Human scFv |
| TAB-448CT-S(P) | Human Anti-Siglec15 Recombinant Antibody; scFv Fragment (TAB-448CT-S(P)) | WB, ELISA, FC | Human scFv |
| TAB-447CT-F(E) | Human Anti-Siglec15 Recombinant Antibody; Fab Fragment (TAB-447CT-F(E)) | WB, ELISA, FC | Human Fab |
| TAB-448CT-F(E) | Human Anti-Siglec15 Recombinant Antibody; Fab Fragment (TAB-448CT-F(E)) | WB, ELISA, FC | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0362MC | Rabbit Anti-Human SIGLEC15 Antibody | WB, IHC-P, P-ELISA |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0039-WJ | Human Anti-SIGLEC15 Recombinant Antibody (HPAB-0039-WJ) | ELISA, WB | Human IgG |
| HPAB-0040-WJ | Human Anti-SIGLEC15 Recombinant Antibody (HPAB-0040-WJ) | ELISA, WB | Humanized IgG |
| HPAB-AP449-YC | Rat Anti-SIGLEC15 Recombinant Antibody (clone 24A3) | Inhib, FuncS | Rat IgG |
| HPAB-AP450-YC | Human Anti-SIGLEC15 Recombinant Antibody (clone ch24A3) | Inhib, FuncS | Chimeric (rat/human) IgG2 |
| HPAB-AP451-YC | Human Anti-SIGLEC15 Recombinant Antibody (clone h24A3-H2/L1) | Inhib, FuncS | Humanized (rat/human) IgG2 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0039-WJ-S(P) | Human Anti-SIGLEC15 Recombinant Antibody; scFv Fragment (HPAB-0039-WJ-S(P)) | ELISA, WB | Human scFv |
| HPAB-0040-WJ-S(P) | Human Anti-SIGLEC15 Recombinant Antibody; scFv Fragment (HPAB-0040-WJ-S(P)) | ELISA, WB | Human scFv |
| HPAB-AP458-YC-S(P) | Human Anti-SIGLEC15 Recombinant Antibody (clone h24A3-H2c/L2b); scFv Fragment | Inhib, FuncS | Humanized (rat/human) scFv |
| HPAB-AP459-YC-S(P) | Human Anti-SIGLEC15 Recombinant Antibody (clone h24A3-H2c/L3b); scFv Fragment | Inhib, FuncS | Humanized (rat/human) scFv |
| HPAB-AP460-YC-S(P) | Human Anti-SIGLEC15 Recombinant Antibody (clone h24A3-H2d/L1); scFv Fragment | Inhib, FuncS | Humanized (rat/human) scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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