Mouse Anti-ssDNA Recombinant Antibody (clone BV04-01) (CAT#: PABZ-115)

Figure 1 Inhibition of horseradish peroxidase-labeled mAb fragments, and SCA 04-01.

Polystyrene plates were coated with ssDNA and co-incubated with horseradish peroxidase-BV04-01 and the following inhibitors, mAb BV04-01 (triangles), BV04-01 Fab fragments (circles), and SCA 04-01 (squares).

Rumbley, C. A., Denzin, L. K., Yantz, L. S. Y. T., Tetin, S. Y., & Voss, E. W. (1993). Construction, characterization, and selected site-specific mutagenesis of an anti-single-stranded DNA single-chain autoantibody. Journal of Biological Chemistry, 268(18), 13667-13674.

Figure 2 Anti-ssDNA activity of BiSCA protein determined in the ELISA.

Individual points represent mean values of triplicate trials with standard deviations (error bars). Points lacking error bars indicate standard deviations smaller than symbol. A, anti-ssDNA activity of fluorescein-purified BiSCA. Polystyrene wells were coated with ssDNA and incubated with BiSCA (10 μg/ml starting concentration) liganded with fluorescein. BiSCA binding to ssDNA was detected with 1A4 (non-ligand-inhibitable anti-4-4-20 antibody) (circles) or 1B7 (ligand-inhibitable anti-4-4-20 antibody) (1 μg/ml) (open triangles). BiSCA was compared to mAb 04-01 (crosses; solid line) and mAb 4-4-20 (closed triangles) (1 μg/ml starting concentration) detected with protein A-HRP. B, anti-ssDNA activity of ssDNA-purified BiSCA (triangles) as compared to equal molar concentrations (1 x 10⁻⁷ M) mAb 04-01 (crosses; solid line). mAb 4-4-20 was hibition of mAb 04-01 and BiSCA binding used as a negative control (circles). C, into ssDNA by soluble DNA. Singlestranded DNA-coated polystyrene wells were incubated with equal molar concentrations(1 x 10⁻⁷ M) of BiSCA (crosses; solid line) and mAb 04-01 (triangles) after preincubation overnight with calf thymus DNA (0.5 mg/ml starting concentration). Individual points represent mean values of duplicate trials.

Mallender, W. D., & Voss, E. W. (1994). Construction, expression, and activity of a bivalent bispecific single-chain antibody. Journal of Biological Chemistry, 269(1), 199-206.