Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody (14G8) (CAT#: PABL-705)
Recombinant Mouse Antibody (14G8) is capable of binding to Staphylococcal enterotoxin B (SEB) , expressed in Chinese Hamster Ovary cells (CHO).
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Figure 1 ELISA shows the effect of binding using different site directed mutagenesis proteins.
Mutant proteins were coated in polystyrene plates at a concentration of 0.5 ug/ml. Further mAb 20B1 or 14G8 or 6D3 or 4C7 was added, detected by alkaline phosphatase (AP)-conjugated goat anti-mouse IgG1 and developed by PNPP tablets. The x -axis represents absorbance at 405 nm and y -axis represents the log of antibody concentration. Results identify different critical residues, which could interact with the individual SEB specific mAbs. For mAb 20B1 mutation of residue 135-R, 137-F, 186-Y, 235 & 236-T affected binding. The residues 135-R, 186-Y were required for the interaction with mAb 6D3. mAb 14G8 bind to residues 135-R, 137-F, 186-Y, 188-K, 231-E, 233-Y, and 235, 236-T, whereas mAb 4C7 interact with 135-R, 137-F, 186-Y, 188-K, and 235, 236-T.
Varshney, A. K. , Wang, X. , Cook, E. , Dutta, K. , & Fries, B. C. . (2011). Generation, characterization, and epitope mapping of neutralizing and protective monoclonal antibodies against staphylococcal enterotoxin b-induced lethal shock. Journal of Biological Chemistry, 286(11), 9737-9747.
Figure 2 Inhibition of T-cell proliferation and cytokine production by treatment with SEB specific mAb 20B1, 14G8, 6D3, and 4C7 individually or in different combinations.
A , SEB-induced T-cell proliferation was measured by ViaLight HS Cell Proliferation kit after 48 h ( A ) and 96 h ( B ) and inhibited in the presence of all three mAb except 4C7. IFN ( C ) and IL-2 ( D ) were measured by ELISA in the supernatant of SEB stimulated T-cells. Cytokines were significantly lower in the presence of mAbs relative to conditions with no specific antibody. The bars represent the S.D. derived from triplicate wells from same experiment.
Varshney, A. K. , Wang, X. , Cook, E. , Dutta, K. , & Fries, B. C. . (2011). Generation, characterization, and epitope mapping of neutralizing and protective monoclonal antibodies against staphylococcal enterotoxin b-induced lethal shock. Journal of Biological Chemistry, 286(11), 9737-9747.
Figure 3 Dot blot analysis of 14G8.
Dot blot analysis shows binding of 10-mer peptide with all three mAbs with SEB and mutant-1 and no binding with mutant-2.
Varshney, A. K. , Wang, X. , Cook, E. , Dutta, K. , & Fries, B. C. . (2011). Generation, characterization, and epitope mapping of neutralizing and protective monoclonal antibodies against staphylococcal enterotoxin b-induced lethal shock. Journal of Biological Chemistry, 286(11), 9737-9747.
Specifications
- Immunogen
- S. aureus Staphylococcal enterotoxin B
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Staphylococcal enterotoxin B (SEB)
- Species Reactivity
- S. aureus
- Clone
- 14G8
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The SEB antibody has been reported in applications of ELISA, Inhibition, Dot, ELISA, Inhibition.
Target
- Alternative Names
- Ent B; EntB; Enterotoxin type B; SEB; Staphylococcal enterotoxin B
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-895 | Recombinant Anti-SEB Antibody | ELISA, IF, FuncS | IgG |
| PABL-336 | Recombinant Human Anti-Staphylococcal enterotoxin B (SEB) Antibody (3E2) | WB, ELISA, Neut, FuncS | IgG |
| PABL-337 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody (20B1) | Neut, FuncS | IgG |
| MOB-0205MZ | Recombinant Mouse Anti-Staphylococcus aureus Enterotoxin B Antibody (clone A287-12288) | ELISA | Mouse antibody |
| MOB-0872CT | Recombinant Mouse anti-Staphylococcus aureus seb Monoclonal antibody (T333) | sELISA |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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