Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody (3E2) (CAT#: PABL-707)

Recombinant Mouse Antibody (3E2) is capable of binding to Staphylococcal enterotoxin B (SEB) , expressed in Chinese Hamster Ovary cells (CHO). This is a neutralizing mAb that recognizes SEB with high specificity and sub-nanomolar affinity. It strongly inhibits SEB-induced T cell proliferation and activation.

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Figure 1 Fluorescence photomicrographs showing the effects of various fusion proteins and monoclonal antibody controls.

(A) Representative image of C. parvum sporozoites after a 30-min exposure to either PBS, the CHO cell supernatant, 4H9-G1, 4H9-G2b, the 18.44 MAb, 4H9-G1- PLA2, 3E2-G1, or the 3E2 MAb. (B) Representative image of C. parvum sporozoites after a 30-min exposure to either 4H9-G2b-LL37, 4H9-G1- LL37, 3E2-G1-LL37, 3E2-Mhalf-LL37, or 3E2-Mmono-LL37. (C) C. parvum sporozoites after exposure to 18.44-G1-PLA2 for 30 min. (D) Heatkilled sporozoites. Viability or cell death was determined by the addition of fluorescein diacetate (green) (live cells) and propidium iodide (red) (dead cells). Each component was used at 25 ug/ml.

Imboden, M., Riggs, M. W., Schaefer, D. A., Homan, E. J., & Bremel, R. D. (2010). Antibodies fused to innate immune molecules reduce initiation of Cryptosporidium parvum infection in mice. Antimicrobial agents and chemotherapy, 54(4), 1385-1392.


Specifications

  • Immunogen
  • S. aureus Staphylococcal enterotoxin B
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native Staphylococcal enterotoxin B (SEB)
  • Species Reactivity
  • S. aureus
  • Clone
  • 3E2
  • Applications
  • ELISA, WB

Applications

  • Application Notes
  • The SEB antibody has been reported in applications of IF.

Target

  • Alternative Names
  • Ent B; EntB; Enterotoxin type B; SEB; Staphylococcal enterotoxin B

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "SEB"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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