Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody (3E2) (CAT#: PABL-707)
Recombinant Mouse Antibody (3E2) is capable of binding to Staphylococcal enterotoxin B (SEB) , expressed in Chinese Hamster Ovary cells (CHO). This is a neutralizing mAb that recognizes SEB with high specificity and sub-nanomolar affinity. It strongly inhibits SEB-induced T cell proliferation and activation.
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Figure 1 Fluorescence photomicrographs showing the effects of various fusion proteins and monoclonal antibody controls.
(A) Representative image of C. parvum sporozoites after a 30-min exposure to either PBS, the CHO cell supernatant, 4H9-G1, 4H9-G2b, the 18.44 MAb, 4H9-G1- PLA2, 3E2-G1, or the 3E2 MAb. (B) Representative image of C. parvum sporozoites after a 30-min exposure to either 4H9-G2b-LL37, 4H9-G1- LL37, 3E2-G1-LL37, 3E2-Mhalf-LL37, or 3E2-Mmono-LL37. (C) C. parvum sporozoites after exposure to 18.44-G1-PLA2 for 30 min. (D) Heatkilled sporozoites. Viability or cell death was determined by the addition of fluorescein diacetate (green) (live cells) and propidium iodide (red) (dead cells). Each component was used at 25 ug/ml.
Imboden, M., Riggs, M. W., Schaefer, D. A., Homan, E. J., & Bremel, R. D. (2010). Antibodies fused to innate immune molecules reduce initiation of Cryptosporidium parvum infection in mice. Antimicrobial agents and chemotherapy, 54(4), 1385-1392.
Specifications
- Immunogen
- S. aureus Staphylococcal enterotoxin B
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Staphylococcal enterotoxin B (SEB)
- Species Reactivity
- S. aureus
- Clone
- 3E2
- Applications
- ELISA, WB
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The SEB antibody has been reported in applications of IF.
Target
- Alternative Names
- Ent B; EntB; Enterotoxin type B; SEB; Staphylococcal enterotoxin B
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-895-F(E) | Recombinant Human Anti-SEB Antibody Fab Fragment | WB, IP, FuncS | Fab |
| PFBL-698 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (14G8) | WB, ELISA, FuncS | Fab |
| PFBL-699 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (6D3) | WB, IF, FuncS | Fab |
| PFBL-700 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (3E2) | ELISA, WB | Fab |
| HPAB-0090-FY-F(E) | Recombinant Mouse Anti-SEB Antibody Fab Fragment (FMU-SEB-No.2) | E |
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