Hamster Anti-TCR Recombinant Antibody (clone H57) (CAT#: TAB-558CT)

Figure 1 Induction of open CD3 in thymocytes stimulated with anti-TCR–CD3 antibodies.

The open CD3 PD assay was performed on lysates from C57BL6 thymocytes that had been incubated in the absence (Ø) or presence of 10 μg/ml anti-TCR (H57) for 5 min at 37 °C.

Gil, D., Schrum, A. G., Alarcón, B., & Palmer, E. (2005). T cell receptor engagement by peptide–MHC ligands induces a conformational change in the CD3 complex of thymocytes. Journal of Experimental Medicine, 201(4), 517-522.

Figure 2 The open CD3 recapture assay was performed on lysates from C57BL6 thymocytes that had been incubated with 10 μg/ml anti-TCR (H57) for 15 min at 37 C.

After the open CD3 complexes had been eluted and recaptured on APA1/1 beads, aliquots of the beads were separately stained with a PE-conjugated mAb probe as indicated and analyzed by flow cytometry.

Gil, D., Schrum, A. G., Alarcón, B., & Palmer, E. (2005). T cell receptor engagement by peptide–MHC ligands induces a conformational change in the CD3 complex of thymocytes. Journal of Experimental Medicine, 201(4), 517-522.