Anti-Human TCR Recombinant Antibody (KJ1-26) (CAT#: TAB-559CT)

Figure 1 Gating of OVA-specific CD4⁺ T cells.

Dead cells were excluded by FVS450 staining. OVA-specific CD4⁺ T cells were gated as CD4⁺ KJ1-26⁺ among live cells.

Ding, Z., Dahlin, J. S., Xu, H., & Heyman, B. (2016). IgE-mediated enhancement of CD4+ T cell responses requires antigen presentation by CD8α− conventional dendritic cells. Scientific reports, 6, 28290.

Figure 2 The other half of each spleen was frozen and spleen sections were stained and analysed by confocal microscopy.

DO11.10 TCR (clone KJ1-26) mAb, red. Images show T cell areas (640 μm × 640 μm) representative of 6 T cell zones from 2 non-consecutive sections per sample in each group. Scale bar represents 100 μm.

Ding, Z., Dahlin, J. S., Xu, H., & Heyman, B. (2016). IgE-mediated enhancement of CD4+ T cell responses requires antigen presentation by CD8α− conventional dendritic cells. Scientific reports, 6, 28290.

Figure 3 Impaired proliferation of KJ1-26⁺ CD4⁺ cells recovered from Ld-nOVA recipients in readoptive transfer experiments into wild-type mice.

On day 14 after the transfer, CD4⁺ T cells were collected from the spleens of the recipients and adoptively transferred into wild-type mice. Wild-type recipients were simultaneously injected with OVA/IFA. Five days after readoptive transfer, draining lymph node cells were collected from the recipients and stainedwith anti-CD4 mAb and KJ1-26.

Kawahata, K., Misaki, Y., Yamauchi, M., Tsunekawa, S., Setoguchi, K., Miyazaki, J. I., & Yamamoto, K. (2002). Peripheral tolerance to a nuclear autoantigen: dendritic cells expressing a nuclear autoantigen lead to persistent anergic state of CD4+ autoreactive T cells after proliferation. The Journal of Immunology, 168(3), 1103-1112.