Anti-Human TCR Recombinant Antibody (H28-710) (CAT#: TAB-561CT)

Figure 1 Quantity of the 55-kDa GIF in culture supernatant of anti-CD3-stimulated 211a cells was much more than that present in cell lysate.

A 20 μl volume of each sample was applied to one lane of SDS/PAGE and analyzed by immunoblotting with mAb H28-710. The cell lysate contained a large quantity of the inactive 13-kDa GIF that nonspecifically bound to Affi-Gel 10, but no 55-kDa GIF.

Ishii, Y., Nakano, T., & Ishizaka, K. (1996). Cellular mechanisms for the formation of a soluble form derivative of T-cell receptor alpha chain by suppressor T cells. Proceedings of the National Academy of Sciences, 93(14), 7207-7212.

Figure 2 Kinetics of the formation of the 55-kDa GIF by the stable transfectant F12 clone.

Aliquots of anti-CD3-treated F12 cells were cultured from 0 to 48 hr. Histidine-rich peptides in culture supernatants were purified using Ni-NTA agarose, and analyzed by SDS/PAGE, followed by immunoblotting with the mAb H28-710. The amount of each sample applied to one lane of SDS/PAGE was derived from 2 × 10⁵ cells.

Ishii, Y., Nakano, T., & Ishizaka, K. (1996). Cellular mechanisms for the formation of a soluble form derivative of T-cell receptor alpha chain by suppressor T cells. Proceedings of the National Academy of Sciences, 93(14), 7207-7212.