Human Anti-TFPI Recombinant Antibody (clone mAb 2021)
CAT#: PABL-718
Recombinant Human Antibody (mAb 2021) is capable of binding to TFPI, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-TFPI mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-TFPI proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This high-affinity (K(D) = 25pM) mAb against TFPI was investigated. Binding of mAb 2021 to TFPI effectively prevented inhibition of FVIIa/TF/FXa and improved clot formation in hemophilia blood and plasma.
Published Data
Gene Expression
Figure 1 Hemostatic effect of mAb 2021 in hemophilia-like plasma and blood.
Hemophilia-like conditions were induced by incubation of normal human plasma or blood with 100 ug/mL FVIII Ab (sheep anti–human FVIII; Hematologic Technologies Inc) for 30 minutes at room temperature. (A) dPT clot analysis with various concentrations of mAb 2021 spiked into normal human plasma (E) and into FVIII-neutralized plasma (F). Results are presented as mean SD (n 3). (B-C) Clot time (B) and clot development values (C) obtained by TEG analysis when various concentrations of mAb 2021 were spiked into FVIII-neutralized human blood. Data are presented as results from individual donors (donor 1:Œ, donor 2:f, donor 3:F, donor 4: ). In the absence of mAb 2021 and anti-FVIII Ab the mean ( SD) clot time obtained in normal blood from the 4 donors was 12.4 3.8 minutes and the clot development was 6.8 1.2 mm 100 s1.
Hilden, I., Lauritzen, B., Sørensen, B. B., Clausen, J. T., Jespersgaard, C., Krogh, B. O., ... & Petersen, H. H. (2012). Hemostatic effect of a monoclonal antibody mAb 2021 blocking the interaction between FXa and TFPI in a rabbit hemophilia model. Blood, blood-2012.
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Normal Tissue
Figure 1 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows distinct positivity in endothelial.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5575/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows distinct cytoplasmic positivity in endothelium.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20842/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20842/46624_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20842/46624_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20842/46624_A_4_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20842/46624_A_2_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, HUVEC TERT2, LHCN-M2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000003436-TFPI
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Specifications
- Immunogen
- Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human TFPI
- Species Reactivity
- Human
- Clone
- mAb 2021
- Applications
- Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The TFPI antibody has been reported in applications of Neutralization. Concentration-dependent neutralization of TFPI inhibition of the FVIIa/TF/FXa complex was performed by direct incubation of FX (160 nM), TFPI (1 nM), TF Innovin (10 pM), and various concentrations of mAb 2021 in a calcium-containing buffer (5 mM) for 15 minutes.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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| MOB-3593z | Mouse Anti-TFPI Recombinant Antibody (clone 41B4) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| HPAB-0476-CN | Mouse Anti-TFPI Recombinant Antibody (HPAB-0476-CN) | ELISA | Mouse IgG |
| HPAB-0477-CN | Human Anti-TFPI Recombinant Antibody (HPAB-0477-CN) | ELISA, Neut | Chimeric (mouse/human) IgG1, κ |
| HPAB-0478-CN | Human Anti-TFPI Recombinant Antibody (HPAB-0478-CN) | ELISA, Neut | Humanized IgG1, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-734 | Anti-TFPI Recombinant Antibody (Concizumab) | ELISA, IP, FC, FuncS, Neut, IF, WB | IgG4 - kappa |
| CAT | Product Name | Application | Type |
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| MOR-3547 | Rabbit Anti-TFPI Recombinant Antibody (clone DS3547AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0488-CN-S(P) | Human Anti-TFPI Recombinant Antibody; scFv Fragment (HPAB-0488-CN-S(P)) | ELISA, FC | Human scFv |
| HPAB-0489-CN-S(P) | Human Anti-TFPI Recombinant Antibody; scFv Fragment (HPAB-0489-CN-S(P)) | ELISA, FC | Human scFv |
| PSBC-461 | Mouse Anti-TFPI Recombinant Antibody; scFv Fragment (PSBC-461) | ELISA, FC, Neut | Mouse scFv |
| HPAB-2580LY-S(P) | Human Anti-TFPI Recombinant Antibody; scFv Fragment (HPAB-2580LY-S(P)) | ELISA, WB, FuncS | Human scfv |
| HPAB-2581LY-S(P) | Human Anti-TFPI Recombinant Antibody; scFv Fragment (HPAB-2581LY-S(P)) | ELISA, WB, FuncS | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-734 | Afuco™ Anti-TFPI ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-734) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2587LY-F(E) | Human Anti-TFPI Recombinant Antibody; Fab Fragment (HPAB-2587LY-F(E)) | ELISA, WB, FuncS | Human Fab |
| HPAB-2588LY-F(E) | Human Anti-TFPI Recombinant Antibody; Fab Fragment (HPAB-2588LY-F(E)) | ELISA, WB, FuncS | Human Fab |
| HPAB-2589LY-F(E) | Human Anti-TFPI Recombinant Antibody; Fab Fragment (HPAB-2589LY-F(E)) | ELISA, WB, FuncS | Human Fab |
| HPAB-2590LY-F(E) | Human Anti-TFPI Recombinant Antibody; Fab Fragment (HPAB-2590LY-F(E)) | ELISA, WB, FuncS | Human Fab |
| HPAB-2591LY-F(E) | Human Anti-TFPI Recombinant Antibody; Fab Fragment (HPAB-2591LY-F(E)) | ELISA, WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC647 | AbPlus™ Anti-TFPI Magnetic Beads (VS-0724-YC647) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0425-FY189 | Human Anti-TFPI (clone Concizumab) scFv-Fc Chimera | ELISA, FC | Human IgG4, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7198 | Anti-TFPI Immunohistochemistry Kit | IHC |
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