Anti-TFRC immunotoxin 454A12 (IgG)-PE
CAT#: AGTO-G068E
This immunotoxin was achieved by conjugating/fusing the Anti-TFRC monoclonal antibody to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating leptomeningeal carcinomatosis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg
Normal Tissue
Figure 7 Bone marrow
Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
❮
❯
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 454A12
- Molecule Class
- IgG-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Leptomeningeal carcinomatosis
Target
- Alternative Names
- TFRC; transferrin receptor; T9; TR; TFR; p90; CD71; TFR1; TRFR; transferrin receptor protein 1; transferrin receptor (p90, CD71); Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE94
- Gene ID
- 7037
- UniProt ID
- P02786
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for AGTO-G068E. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 454A12"
- CAT
- Product Name
See other products for "TFRC"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1176z-F(E) | Recombinant Anti-Human TFRC Antibody Fab Fragment | WB, Neut, FuncS | Fab |
| HPAB-0376-CN-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0376-CN-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0377-CN-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0377-CN-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-0378-CN-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0378-CN-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-2579LY-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-2579LY-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1176z-S(P) | Recombinant Anti-Human TFRC Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| HPAB-0376-CN-S(P) | Mouse Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-0376-CN-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-0238-YJ-S(P) | Rat Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-0238-YJ-S(P)) | IHC, FC, WB, FuncS | Rat scFv |
| HPAB-0239-YJ-S(P) | Rat Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-0239-YJ-S(P)) | IHC, FC, WB, FuncS | Rat scFv |
| HPAB-0240-YJ-S(P) | Rat Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-0240-YJ-S(P)) | IHC, FC, WB, FuncS | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G067E | Anti-TFRC immunotoxin HB21 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G067D | Anti-TFRC immunotoxin HB21 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G067G | Anti-TFRC immunotoxin HB21 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G067R | Anti-TFRC immunotoxin HB21 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G067S | Anti-TFRC immunotoxin HB21 (scFv)-Sap | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0467CL-S(P) | Human Anti-TFRC Recombinant Antibody; scFv Fragment (TAB-0467CL-S(P)) | FuncS | Human scFv |
| TAB-0467CL-F(E) | Human Anti-TFRC Recombinant Antibody; Fab Fragment (TAB-0467CL-F(E)) | FuncS | Human Fab |
| TAB-619CT | Human Anti-TFRC Recombinant Antibody (TAB-619CT) | ELISA, WB | Human antibody |
| TAB-620CT | Human Anti-TFRC Recombinant Antibody (TAB-620CT) | ELISA, WB | Human antibody |
| TAB-621CT | Anti-Human TfR Recombinant Antibody (LUCA31) | ELISA, WB, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-611CT | Humanized Anti-TFRC Recombinant Antibody (TAB-611CT) | ELISA | Humanized IgG |
| TAB-611CT-S(P) | Humanized Anti-TFRC Recombinant Antibody; scFv Fragment (TAB-611CT-S(P)) | ELISA | Humanized scFv |
| TAB-612CT-S(P) | Humanized Anti-TFRC Recombinant Antibody; scFv Fragment (TAB-612CT-S(P)) | ELISA | Humanized scFv |
| TAB-613CT-S(P) | Humanized Anti-TFRC Recombinant Antibody; scFv Fragment (TAB-613CT-S(P)) | ELISA | Humanized scFv |
| TAB-614CT-S(P) | Humanized Anti-TFRC Recombinant Antibody; scFv Fragment (TAB-614CT-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-615CT | Anti-Human TfR Recombinant Antibody (6-90) | ELISA, WB, IP |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-616CT | Anti-Human TfR Recombinant Antibody (55-31) | ELISA, WB, IP | |
| TAB-617CT | Anti-Human TfR Recombinant Antibody (122-72) | ELISA, WB, IP | |
| TAB-618CT | Anti-Human TfR Recombinant Antibody (5D7-54.17) | ELISA, WB, IP | |
| TAB-615CT-F(E) | Anti-Human TfR Recombinant Antibody Fab Fragment (6-90) | ELISA, WB, IP | |
| TAB-616CT-F(E) | Anti-Human TfR Recombinant Antibody Fab Fragment (55-31) | ELISA, WB, IP |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0036MZ | Recombinant Mouse Anti-Human TFRC Antibody (clone A8-2) | ELISA, WB | Mouse antibody |
| HPAB-0376-CN | Mouse Anti-TFRC Recombinant Antibody (HPAB-0376-CN) | ELISA, FC | Mouse IgG |
| HPAB-0377-CN | Mouse Anti-TFRC Recombinant Antibody (HPAB-0377-CN) | ELISA, FC | Mouse IgG |
| HPAB-0378-CN | Mouse Anti-TFRC Recombinant Antibody (HPAB-0378-CN) | ELISA, FC | Mouse IgG |
| HPAB-0379-CN | Human Anti-TFRC Recombinant Antibody (HPAB-0379-CN) | ELISA, FC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1999CQ | Rat Anti-Tfrc Recombinant Antibody (clone RI7217) | FC, ICC, IP, WB, Block | Rat IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3548 | Rabbit Anti-TFRC Recombinant Antibody (clone DS3548AB) | FC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3370 | PE-DRB1*13:02/Human TFRC (NPGGYVAYSKAATVTGKL) MHC Tetramer | FCM | |
| MHC-LC3371 | PE-DRB1*13:02/Human TFRC (NPGGYVAYSKAATVTG) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1481 | AbPlus™ Anti-TFRC Magnetic Beads (VS-0724-YC1481) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY205 | CytoStream™ Mouse Anti-TFRC Recombinant Antibody (clone OX-26) | FC, IHC, IP | Mouse IgG2a, kappa |
| VS-0225-XY206 | CytoStream™ Rat Anti-TFRC Recombinant Antibody (clone RI7217) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC571 | Recombinant Anti-TFRC Vesicular Antibody, EV Displayed (VS-0425-YC571) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7200 | Anti-Mouse TFRC Immunohistochemistry Kit | IHC | |
| VS-0525-XY7201 | Anti-Rat TFRC Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FuncS, Inhib, IP, ELISA
-2.png)
Application: WB, ELISA, FC, IHC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
