Mouse Anti-TGFA Recombinant Antibody (clone tAb2) (CAT#: PABZ-122)

Recombinant Mouse Antibody (tAb2) is capable of binding to TGF Alpha, expressed in Chinese Hamster Ovary cells (CHO).


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Figure 1 Effect of anti-human TGFa monoclonal antibodies on the anchorage-independent growth of NOG-8 TF CI 5 and 7 cells.

Figure 1 Effect of anti-human TGFa monoclonal antibodies on the anchorage-independent growth of NOG-8 TF CI 5 and 7 cells.

Cells (2 x 10⁴ were seeded in 0.3% agar containing DMEM and 10% FBS in 35-mm dishes and treated with different concentrations of mouse monoclonal anti-TGFa antibody TAbl or TAb2. The cells were grown for 14 d, stained with nitro blue tetrazolium, and counted with an Artek colony counter. (A) NOG-8 TF CI 5 cells; colonies greater than 150 pm for the untreated control cultures were 1022 colonies/dish. (B) NOG-8 TF CI 7 cells; colonies greater than 150 km for the untreated control cultures were 11 18 coloniesldish. Data are expressed as the percentage of cell growth as colonies in the presence of different concentrations of TAbl or TAb2 compared to untreated controls. Values are the average (± SD) of triplicate determinations.

Shankar, V., Ciardiello, F., Kim, N., Derynck, R., Liscia, D. S., Merlo, G., ... & Lippman, M. E. (1989). Transformation of an established mouse mammary epithelial cell line following transfection with a human transforming growth factor alpha cDNA. Molecular carcinogenesis, 2(1), 1-11.

Figure 2 Western blotting.

Figure 2 Western blotting.

Equal amounts of protein isolated from diierent ovarian tumours and non-malignant tissue as described above were resolved on an SDS-polyacrylamide gel and blotted onto a nitrocefhdose filter. TAB2 antibody was diluted 1:1000 and biotinylated rabbit anti-mouse and streptavidin peroxidase conjugate were employed to visualise immunospecilic bands. Molecular weight markers are indicated on the letI side of the figure. (a) lane 1: 363, 2: 378,3: 480,4: 483,5: 513,6: non-malignant tissue. (b) lane 1: 573,2: 506,3: 138,4:448.

Kohler, M., Bauknecht, T., Grimm, M., Birmelin, G., Kommoss, F., & Wagner, E. (1992). Epidermal growth factor receptor and transforming growth factor alpha expression in human ovarian carcinomas. European Journal of Cancer, 28(8), 1432-1437.


Specifications

  • Immunogen
  • Human transforming growth factor, alpha
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human TGFA
  • Species Reactivity
  • Human
  • Clone
  • tAb2
  • Applications
  • WB, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody tAb2 has been reported in applications of FuncS, WB. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • TGFA; transforming growth factor, alpha; TFGA; protransforming growth factor alpha; TGF-alpha;

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone tAb2"

See other products for "TGFA"

Recombinant Antibody

CAT Product Name Application Type
MOB-2017CT Recombinant Mouse anti-Human TGFA Monoclonal antibody (Q/U2) ELISA, ICC, IHC-Fr, IHC-P
HPAB-0544-CN Mouse Anti-TGFA Recombinant Antibody (HPAB-0544-CN) WB, ELISA, FC, IHC, IP, Neut Mouse IgG1, κ
HPAB-0545-CN Mouse Anti-TGFA Recombinant Antibody (HPAB-0545-CN) WB, ELISA, FC, IHC, IP, Neut Mouse IgG1, κ
HPAB-0546-CN Mouse Anti-TGFA Recombinant Antibody (HPAB-0546-CN) WB, ELISA, FC, IHC, IP, Neut Mouse IgG2a, κ
VS3-FY1445 Recombinant Rabbit Anti-TGFA Antibody (clone R07-6J8) WB, IHC-P, IP Rabbit IgG

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