How can I use this antibody in my research?

This antibody is great for studying TIM3 in different ways. You can use it in flow cytometry to see if TIM3 is on the surface of cells. You can also use it in functional studies, like mixed lymphocyte reactions (MLR), to see how it affects the immune response. It's like having a tool to tweak the immune system and see how it reacts, which can help you understand more about cancer and other diseases.

How pure is this antibody?

This antibody is very pure! It's more than 95% pure, which means it's almost all the antibody you need and very little of anything else. This is important because it helps you get really accurate results in your experiments. When you use a pure antibody, you don't have to worry about other things getting in the way of what you're trying to find.

Anti-Human TIM3 Recombinant Antibody (TSR-022) (CAT#: TAB-0366CL)

The TIM-3-specific antibody demonstrates potent and durable anti-tumor activity in syngeneic tumor models and therefore can be used for immunotherapy.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Published Data
Tested Data
Datasheet
MSDS
COA

Figure 1 TSR-022 binding to native TIM-3.

Figure 1 TSR-022 binding to native TIM-3.

TSR022 binding to monocytes that were purified from peripheral blood mononuclear cells (PBMCs) was confirmed.

Murtaza, A., Laken, H., Correia, J. D. S., McNeeley, P., Altobell, L., Zhang, J., ... & Jenkins, D. (2016). Discovery of TSR-022, a novel, potent anti-human TIM-3 therapeutic antibody. European Journal of Cancer, 69, S102.

FuncS

Figure 2 MLR assay.

Figure 2 MLR assay.

The functional activity of TSR-022 was also evaluated in a mixed lymphocyte reaction (MLR) assay, in which primary human CD4+ T-cells were mixed with monocyte-derived dendritic cells from a different donor.

ELISA

Figure 1 Human TIM3 Recombinant Antibody (TAB-0366CL) in ELISA

Figure 1 Human TIM3 Recombinant Antibody (TAB-0366CL) in ELISA

ELISA analysis of TAB-0366CL was performed by coating with Recombinant Human TIM3, His tag. Then blocked with BSA, and incubated with Anti-Human TIM3 Antibody (TAB-0366CL). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Human TIM3 Recombinant Antibody (TAB-0366CL) in WB

Figure 2 Human TIM3 Recombinant Antibody (TAB-0366CL) in WB

Western blot analysis of TAB-0366CL was performed with Recombinant Human TIM3, His tag in non-reduced (lane 1, 0.5 μg) and reduced condition (lane 2, 0.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0366CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human TIM-3 (for panning)

Host Species
Human

Type
Human antibody

Specificity
Human

Clone
TSR-022

Applications
FC, FuncS

Related Disease
Tumor

Applications

Application Notes
The TIM-3 antibody has been reported in applications of Flow Cytometry, Functional Assay.

Target

Alternative Names
T-cell immunoglobulin and mucin domain containing molecule-3; TIM3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Fab Antibody Products ScFv Antibody Products Human Antibody Products Mouse Antibody Products

CAT	Product Name	Application	Type
MOB-976	Recombinant Anti-Tim-3 Antibody	WB, Neut, FuncS	IgG
MHH-976	Recombinant Human Anti-Tim-3 Antibody	ELISA, WB, FuncS	IgG
VS-0723-WK188	Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK188)	ELISA, FC	Rabbit IgG
VS-0723-WK280	Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK280)	ELISA, FC	Rabbit IgG
VS-0723-WK289	Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK289)	ELISA, FC	Rabbit IgG

CAT	Product Name	Application	Type
MOB-976-F(E)	Recombinant Anti-Tim-3 Antibody Fab Fragment	FC, Neut, Funcs	Fab
MHH-976-F(E)	Recombinant Human Anti-Tim-3 Antibody Fab Fragment	ELISA, WB, IP, FuncS	Fab

CAT	Product Name	Application	Type
MOB-976-S(P)	Recombinant Anti-Tim-3 Antibody scFv Fragment	WB, ELISA, IHC, FuncS	scFv
MHH-976-S(P)	Recombinant Human Anti-Tim-3 Antibody scFv Fragment	IP, IF, Biosensors, FuncS	scFv

CAT	Product Name	Application	Type
TAB-0366CL-S(P)	Anti-Human TIM3 Recombinant Antibody scFv Fragment (TSR-022)	ELISA, WB	Human antibody
TAB-0366CL-F(E)	Anti-Human TIM3 Recombinant Antibody Fab Fragment (TSR-022)	ELISA, WB	Human antibody

CAT	Product Name	Application	Type
TAB-665CT-F(E)	Anti-Human TIM-3 Recombinant Antibody Fab Fragment (F38-2E2)	ELISA, FC, Inhibit
TAB-666CT-F(E)	Anti-Human TIM-3 Recombinant Antibody Fab Fragment (mAb15)	ELISA, FC, Inhibit
TAB-667CT-F(E)	Mouse Anti-TIM3 Recombinant Antibody; Fab Fragment (TAB-667CT-F(E))	ELISA, FC, Inhib	Mouse Fab
TAB-668CT-F(E)	Mouse Anti-TIM3 Recombinant Antibody; Fab Fragment (TAB-668CT-F(E))	ELISA, FC, Inhib	Mouse Fab
TAB-669CT-F(E)	Mouse Anti-TIM3 Recombinant Antibody; Fab Fragment (TAB-669CT-F(E))	ELISA, FC, Inhib	Mouse Fab

Customer Reviews and Q&As

Customer Review Q&As

Great for Cancer Research

This antibody works really well for detecting TIM3 on cancer cells. I used it in my experiments to see how well immune cells could attack cancer cells with its help. The results were clear and easy to read. It's very sensitive, so I could detect even small amounts of TIM3. I also like that it's made in an ethical way without harming animals. Overall, it's a great tool for my cancer research.

High Sensitivity

I was really impressed with how sensitive this antibody is. I could detect very small amounts of TIM3 in my samples, which is important for my research. The results were clear and consistent, which makes me trust the data I get. It's a great tool for studying cancer cells.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-0366CL, RRID: AB_3111771)

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