Anti-Human TIM3 Recombinant Antibody (TSR-022)
CAT#: TAB-0366CL
The TIM-3-specific antibody demonstrates potent and durable anti-tumor activity in syngeneic tumor models and therefore can be used for immunotherapy.
Published Data
Tested Data
FC
Figure 1 TSR-022 binding to native TIM-3.
TSR022 binding to monocytes that were purified from peripheral blood mononuclear cells (PBMCs) was confirmed.
Murtaza, A., Laken, H., Correia, J. D. S., McNeeley, P., Altobell, L., Zhang, J., ... & Jenkins, D. (2016). Discovery of TSR-022, a novel, potent anti-human TIM-3 therapeutic antibody. European Journal of Cancer, 69, S102.
FuncS
Figure 2 MLR assay.
The functional activity of TSR-022 was also evaluated in a mixed lymphocyte reaction (MLR) assay, in which primary human CD4+ T-cells were mixed with monocyte-derived dendritic cells from a different donor.
Murtaza, A., Laken, H., Correia, J. D. S., McNeeley, P., Altobell, L., Zhang, J., ... & Jenkins, D. (2016). Discovery of TSR-022, a novel, potent anti-human TIM-3 therapeutic antibody. European Journal of Cancer, 69, S102.
❮
❯
ELISA
Figure 1 Human TIM3 Recombinant Antibody (TAB-0366CL) in ELISA
ELISA analysis of TAB-0366CL was performed by coating with Recombinant Human TIM3, His tag. Then blocked with BSA, and incubated with Anti-Human TIM3 Antibody (TAB-0366CL). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Human TIM3 Recombinant Antibody (TAB-0366CL) in WB
Western blot analysis of TAB-0366CL was performed with Recombinant Human TIM3, His tag in non-reduced (lane 1, 0.5 μg) and reduced condition (lane 2, 0.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0366CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
❮
❯
Specifications
- Immunogen
- Human TIM-3 (for panning)
- Host Species
- Human
- Type
- Human antibody
- Specificity
- Human
- Clone
- TSR-022
- Applications
- FC, FuncS
- Related Disease
- Tumor
Applications
- Application Notes
- The TIM-3 antibody has been reported in applications of Flow Cytometry, Functional Assay.
Target
- Alternative Names
- T-cell immunoglobulin and mucin domain containing molecule-3; TIM3
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for TAB-0366CL. Click the button above to contact us or submit your feedback about this product.
Great for Cancer Research
This antibody works really well for detecting TIM3 on cancer cells. I used it in my experiments to see how well immune cells could attack cancer cells with its help. The results were clear and easy to read. It's very sensitive, so I could detect even small amounts of TIM3. I also like that it's made in an ethical way without harming animals. Overall, it's a great tool for my cancer research.
High Sensitivity
I was really impressed with how sensitive this antibody is. I could detect very small amounts of TIM3 in my samples, which is important for my research. The results were clear and consistent, which makes me trust the data I get. It's a great tool for studying cancer cells.
Q&As
-
How can I use this antibody in my research?
A: This antibody is great for studying TIM3 in different ways. You can use it in flow cytometry to see if TIM3 is on the surface of cells. You can also use it in functional studies, like mixed lymphocyte reactions (MLR), to see how it affects the immune response. It's like having a tool to tweak the immune system and see how it reacts, which can help you understand more about cancer and other diseases.
-
How pure is this antibody?
A: This antibody is very pure! It's more than 95% pure, which means it's almost all the antibody you need and very little of anything else. This is important because it helps you get really accurate results in your experiments. When you use a pure antibody, you don't have to worry about other things getting in the way of what you're trying to find.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-0366CL, RRID: AB_3111771)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone TSR-022"
- CAT
- Product Name
See other products for "TIM3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-976 | Recombinant Anti-Tim-3 Antibody | WB, Neut, FuncS | IgG |
| MHH-976 | Recombinant Human Anti-Tim-3 Antibody | ELISA, WB, FuncS | IgG |
| VS-0723-WK188 | Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK188) | ELISA, FC | Rabbit IgG |
| VS-0723-WK280 | Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK280) | ELISA, FC | Rabbit IgG |
| VS-0723-WK289 | Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK289) | ELISA, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-976-F(E) | Recombinant Anti-Tim-3 Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| MHH-976-F(E) | Recombinant Human Anti-Tim-3 Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-976-S(P) | Recombinant Anti-Tim-3 Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| MHH-976-S(P) | Recombinant Human Anti-Tim-3 Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0366CL-S(P) | Anti-Human TIM3 Recombinant Antibody scFv Fragment (TSR-022) | ELISA, WB | Human antibody |
| TAB-0366CL-F(E) | Anti-Human TIM3 Recombinant Antibody Fab Fragment (TSR-022) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-673CT | Mouse Anti-TIM3 Recombinant Antibody (TAB-673CT) | ELISA, FC, Inhib | Mouse IgG2b, κ |
| TAB-674CT | Mouse Anti-TIM3 Recombinant Antibody (TAB-674CT) | ELISA, FC, Inhib | Mouse IgG1, κ |
| TAB-675CT | Mouse Anti-TIM3 Recombinant Antibody (TAB-675CT) | ELISA, FC, Inhib | Mouse IgG1, κ |
| TAB-676CT | Mouse Anti-TIM3 Recombinant Antibody (TAB-676CT) | ELISA, FC, Inhib | Mouse IgG1, κ |
| TAB-677CT | Mouse Anti-TIM3 Recombinant Antibody (TAB-677CT) | ELISA, FC, Inhib | Mouse IgG1, κ |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: ELISA, WB, BLI, SPR
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, WB, FC, IHC, IP
Application: Neut, IHC, Activ, FuncS, IF, ELISA
Application: ELISA, FC, Neut, Inhib
Application: ELISA, Neut, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
