Clone TPP-2090 is a human monoclonal antibody that binds to TWEAKR. TPP-2090 can be potentially used in the treatment studies of tumors and other disorders and conditions associated with expression of the TWEAKR.
Figure 1 Interaction of TWEAKR ectodomain with antibodies and reference antibodies.
Shown is the result of an ELISA with TWEAKR-Fc fusion protein (TPP-2202) coating (1 mg/ml) and 0.08 mg/ml (open bars) and 0.3 mg/ml (filled bars) of biotinylated IgG as soluble binding partner. Detection was done with Streptavidin-HRP and Amplex-Red substrate. Y is "ELISA signal intensity [Rfu]"; X are "antibody constructs tested": a is "TPP-2090"; b is "TPP-2084"; c is "PDL-192(TPP-1104)"; d is "P4A8(TPP-1324)"; e is "P3G5(TPP-2195)"; f is "136.1(TPP-2194)"; h is "ITEM1"; i is "ITEM4"; j is a murine isotype control; k is a human isotype control. All tested antibodies show saturated binding with a concentration of 80 ng/ml.
Figure 2 Interaction of TWEAKR cysteine rich domain with antibodies and reference antibodies.
Shown is the result of an ELISA with TWEAKR(34-68)-Fc fusion protein (TPP-2203) coating (1 mg/ml) and 0.08 mg/ml (open bars) and 0.3 mg/ml (filled bars) of biotinylated IgG as soluble binding partner. Detection was done with Streptavidin-HRP and Amplex-Red substrate. Y is "ELISA signal intensity [Rfu]"; X are "antibody constructs tested": a is "TPP-2090"; b is "TPP-2084"; c is "PDL-192(TPP-1104)"; d is "P4A8(TPP-1324)"; e is "P3G5(TPP-2195)"; f is "136.1(TPP-2194)"; h is "ITEM1"; i is "ITEM4"; j is a murine isotype control; k is a human isotype control. The antibodies bind to the cysteine rich domain.
Figure 3 Induction of Stat-1 and NF-kappaB2 signaling pathways by anti-TWEAKR antibodies in vivo.
Treatment of mice started at day 7 after tumor cell inoculation and lysates were prepared from snap frozen tumors taken at the end of the study (day 29). Blots from 2 representative animals per group are shown. Treatment with TPP-2090 leads to a strong induction of P-Stat 1 & Total Stat 1 levels as well as NF-kappaB2 activation (shown by the appearance of the p52 band) in WiDr xenograft tumors. Lysates of snap frozen WiDr xenograft tumors after treatment with PBS (i.v., q4dx7: lanes 1&2) or TPP-2090 (3 mg/kg, i.v., q4dx7: lanes 3&4) were subjected to Western Blot analysis detected with specific antibodies for P-Stat 1 (a), Stat-1 (b), NF-kappa2-p52 (c) and GAPDH (d).
Figure 4 Efficacy of 10 mg/kg TPP-2090 (i.v., q4dx8) in the human lung cancer xenograft NCI-H322 in monotherapy and combination therapy with Paclitaxel (16 mg/kg, i.v., q7dx4).
Treatment started 14d after inoculation with established tumors of about 45 mm2. A is "Vehicle group, treated with PBS (i.v. q4dx8)". B is "TPP-2090, 5 mg/kg", C is "TPP-2090, 10 mg/kg", D is "Paclitaxel, 16 mg/kg", E is "Combo TPP-2090 10 mg/kg+Paclitaxel 16 mg/kg". (X is "Time after inoculation [days]"; Y is "Tumor area, means of n=10; SD [mm²]")
Figure 5 Efficacy of anti-TWEAKR antibodies in the human renal cell cancer xenograft 786-O after treatment with 0.3, 1.0 and 3.0 mg/kg (i.v., q4dx3) started at day 7 after tumor cell inoculation.
Shown are final tumor weights at day 40. A is "Vehicle group, treated with PBS (i.v. q4dx3)". B is "Isotype, 3 mg/kg", C is "TPP-2084, 0.3 mg/kg", D is "TPP-2084, 1 mg/kg", E is "TPP-2084, 3 mg/kg", F is "TPP-2090, 0.3 mg/kg", G is "TPP-2090, 1 mg/kg", H is "TPP-2090, 3 mg/kg". (Y is "Tumor weights means of n=8; SD [g]").
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CAT | Product Name | Application | Type |
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AGTO-G061G | Anti-TNFRSF12A immunotoxin ITEM-4 (scFv)-Gel | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-221CQ | Anti-Human TNFRSF12A Recombinant Antibody (TPP-883) | ELISA, FC, WB | Human antibody |
TAB-221CQ-S(P) | Anti-Human TNFRSF12A Recombinant Antibody scFv Fragment (TPP-883) | ELISA, FC, WB | Human antibody |
TAB-221CQ-F(E) | Anti-Human TNFRSF12A Recombinant Antibody Fab Fragment (TPP-883) | ELISA, FC, WB | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-222CQ | Mouse Anti-TNFRSF12A Recombinant Antibody (TAB-222CQ) | Agonist, ELISA, FuncS, WB | Mouse IgG |
TAB-223CQ | Mouse Anti-TNFRSF12A Recombinant Antibody (TAB-223CQ) | Agonist, ELISA, FuncS | Mouse IgG |
TAB-218CQ-S(P) | Mouse Anti-TNFRSF12A Recombinant Antibody; scFv Fragment (TAB-218CQ-S(P)) | ELISA, FC, IHC | Mouse scFv |
TAB-222CQ-S(P) | Mouse Anti-TNFRSF12A Recombinant Antibody; scFv Fragment (TAB-222CQ-S(P)) | ELISA, FC | Mouse scFv |
TAB-223CQ-S(P) | Mouse Anti-TNFRSF12A Recombinant Antibody; scFv Fragment (TAB-223CQ-S(P)) | ELISA, FC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-219CQ-S(P) | Human Anti-TNFRSF12A Recombinant Antibody; scFv Fragment (TAB-219CQ-S(P)) | ELISA, FC, IHC | Humanized scFv |
TAB-225CQ-S(P) | Human Anti-TNFRSF12A Recombinant Antibody; scFv Fragment (TAB-225CQ-S(P)) | ELISA, FC | Humanized scFv |
TAB-227CQ-S(P) | Human Anti-TNFRSF12A Recombinant Antibody; scFv Fragment (TAB-227CQ-S(P)) | ELISA, FC | Humanized scFv |
TAB-219CQ-F(E) | Human Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (TAB-219CQ-F(E)) | ELISA, FC, IHC | Humanized Fab |
TAB-225CQ-F(E) | Human Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (TAB-225CQ-F(E)) | ELISA, FC | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-220CQ-S(P) | Mouse Anti-TNFRSF12A Recombinant Antibody; scFv Fragment (TAB-220CQ-S(P)) | ELISA, FC, IHC | Mouse scFv |
TAB-220CQ-F(E) | Human Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (TAB-220CQ-F(E)) | ELISA, FC, IHC | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-194LC | Recombinant Anti-Human TNFRSF12A Antibody (Non-glycosylated) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-3610 | Rabbit Anti-TNFRSF12A Recombinant Antibody (clone DS3610AB) | IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0048-LSX-S(P) | Human Anti-TNFRSF12A Recombinant Antibody (clone TPP-883); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0049-LSX-S(P) | Human Anti-TNFRSF12A Recombinant Antibody (clone TPP-2090); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0050-LSX-S(P) | Human Anti-TNFRSF12A Recombinant Antibody (clone TPP-2658); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0051-LSX-S(P) | Human Anti-TNFRSF12A Recombinant Antibody (clone TPP-2084); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0052-LSX-S(P) | Human Anti-TNFRSF12A Recombinant Antibody (clone TPP-1104); scFv Fragment | ELISA, WB, FC | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0112LY | Human Anti-TNFRSF12A Recombinant Antibody (HPAB-0112LY) | ELISA | Humanized IgG1 |
HPAB-0113LY | Mouse Anti-TNFRSF12A Recombinant Antibody (HPAB-0113LY) | ELISA | Mouse IgG2a |
HPAB-0114LY | Mouse Anti-TNFRSF12A Recombinant Antibody (HPAB-0114LY) | ELISA | Mouse IgG |
HPAB-0115LY | Mouse Anti-TNFRSF12A Recombinant Antibody (HPAB-0115LY) | ELISA | Mouse IgG |
HPAB-0116LY | Human Anti-TNFRSF12A Recombinant Antibody (HPAB-0116LY) | ELISA | Humanized IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0112LY-F(E) | Human Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (HPAB-0112LY-F(E)) | ELISA | Humanized Fab |
HPAB-0113LY-F(E) | Mouse Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (HPAB-0113LY-F(E)) | ELISA | Mouse Fab |
HPAB-0114LY-F(E) | Mouse Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (HPAB-0114LY-F(E)) | ELISA | Mouse Fab |
HPAB-0115LY-F(E) | Mouse Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (HPAB-0115LY-F(E)) | ELISA | Mouse Fab |
HPAB-0116LY-F(E) | Human Anti-TNFRSF12A Recombinant Antibody; Fab Fragment (HPAB-0116LY-F(E)) | ELISA | Humanized Fab |
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