Mouse Anti-TNFRSF8 Recombinant Antibody (clone T405) (CAT#: HPAB-0447-CN)
T405 is a monoclonal anti-CD30 antibody which is of IgG2b isotype. T405 binds to intact CD30 but not soluble CD30, thus can be used to target cells with intact CD30 without competition from sCD30.
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0447-CN) in SDS-PAGE
SDS-PAGE analysis of HPAB-0447-CN in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180-275 kDa.
SEC-HPLC
Figure 2 Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0447-CN) in SEC-HPLC
The purity of HPAB-0447-CN was greater than 99% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0447-CN) in DB
Dot blot analysis of HPAB-0447-CN was performed by loading Recombinant Human TNFRSF8 (His Tag).
HPAB-0447-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 4 Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0447-CN) in WB
Western blot analysis of HPAB-0447-CN was performed by loading Recombinant Human TNFRSF8 (His Tag).
HPAB-0447-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
ELISA
Figure 5 Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0447-CN) in ELISA
ELISA analysis of HPAB-0447-CN was performed by coating with Recombinant Human TNFRSF8.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Specifications
- Immunogen
- Plasmid cDNA encoding full-length human CD30
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TNFRSF8
- Species Reactivity
- Human
- Clone
- T405
- Applications
- WB, ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-144 | Anti-Human CD30 Recombinant Antibody (Iratumumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-153 | Chimeric (Mouse/Human) Anti-TNFRSF8 Recombinant Antibody (clone Brentuximab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Human IgG1, κ |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G062E | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G062R | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G062S | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-Sap | Cytotoxicity assay, Function study | |
| AGTO-L041R | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L041S | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-Sap | Cytotoxicity assay, Functional assay |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-635CL | Human Anti-TNFRSF8 Recombinant Antibody (TAB-635CL) | ELISA | Human IgG |
| TAB-103LC | Anti-Human TNFRSF8 Recombinant Antibody | WB, FC, ELISA | Humanized antibody |
| TAB-103LC-S(P) | Anti-Human TNFRSF8 Recombinant Antibody scFv Fragment | WB, FC, ELISA | Humanized antibody |
| TAB-103LC-F(E) | Anti-Human TNFRSF8 Recombinant Antibody Fab Fragment | WB, FC, ELISA | Humanized antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-139LC | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
Low- or Non-fucosylated Oligosaccharide
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-139LC-1 | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0282MZ | Recombinant Mouse Anti-Human tumor necrosis factor receptor superfamily, member 8 Antibody (clone Cer-H3) | IHC | Mouse antibody |
| HPAB-0329-YC | Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0329-YC) | ELISA, FC, Cyt, FuncS, Inhib | Mouse IgG |
| HPAB-0330-YC | Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0330-YC) | ELISA, FC, Cyt, FuncS, Inhib | Chimeric (mouse/human) IgG1, κ |
| HPAB-0331-YC | Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0331-YC) | ELISA, FC, Cyt, FuncS, Inhib | Mouse IgG |
| HPAB-0332-YC | Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0332-YC) | FC, Cyt, FuncS | Human IgG1, κ |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0597MZ | Chicken Anti-TNFRSF8 Polyclonal IgY | WB | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2165CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone mCD30.1) | FC, Stim, Costim, Block | Hamster IgG1 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2166CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone 2SH12-5F) | Neut, FC | Hamster IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3617 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3617AB) | WB, IHC-P | Rabbit IgG |
| MOR-3618 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3618AB) | ELISA | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0329-YC-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0329-YC-F(E)) | ELISA, FC, Cyt, FuncS | Mouse Fab |
| HPAB-0330-YC-F(E) | Human Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0330-YC-F(E)) | ELISA, FC, Cyt, FuncS | Human Fab |
| HPAB-0331-YC-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0331-YC-F(E)) | ELISA, FC, Cyt, FuncS | Mouse Fab |
| HPAB-0332-YC-F(E) | Human Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0332-YC-F(E)) | FC, Cyt | Human Fab |
| HPAB-0333-YC-F(E) | Human Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0333-YC-F(E)) | FC, Cyt, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0325CQ-S(P) | Human Anti-TNFRSF8 Recombinant Antibody; scFv Fragment (HPAB-0325CQ-S(P)) | ELISA, FC, FuncS, Cyt | Humanized scFv |
| HPAB-0459-WJ-S(P) | Mouse Anti-TNFRSF8 Recombinant Antibody; scFv Fragment (HPAB-0459-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-1929-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 17G1); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-1930-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 2H9); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-1931-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 5F11); scFv Fragment | FC, ELISA | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-144 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Iratumumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-153 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Brentuximab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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