Human Anti-TNFSF11 Recombinant Antibody (TAB-H18) (CAT#: TAB-H18)
This product is a recombinant human antibody that recognizes TNFSF11. The antibody was purified by affinity chromatography.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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SDS-PAGE
Figure 1 Anti-Human TNFSF11 Antibody (TAB-H18) in SDS-PAGE
SDS-PAGE analysis of TAB-H18 in non-reduced (lane 2) and reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 2 Anti-Human TNFSF11 Antibody (TAB-H18) in HPLC
The purity of TAB-H18 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human TNFSF11 Antibody (TAB-H18) in WB
Western blot analysis of TAB-H18 was performed by loading Human TNFSF11 Protein (His Tag).
TAB-H18 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Anti-Human TNFSF11 Antibody (TAB-H18) in ELISA
ELISA analysis of TAB-H18 was performed by coating with Human TNFSF11 Protein (His Tag). Then blocked with BSA and incubated with TAB-H18. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 5 Anti-Human TNFSF11 Antibody (TAB-H18) in DB
Dot Blot analysis of TAB-H18 was performed by coating with Recombinant Human TNFSF11 Protein (his Tag).
TAB-H18 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Specifications
- Host Species
- Human
- Derivation
- human
- Type
- Human IgG2
- Species Reactivity
- Human
- Applications
- Suitable for use in Neut, ELISA, IF, IP, FuncS, FC, ICC and most other immunological methods.
- MW
- 144.7 kDa
- Related Disease
- Postmenopausal women osteoporosis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
- Gene ID
- 8600
- UniProt ID
- O14788
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1441z | Mouse Anti-TNFSF11 Recombinant Antibody (clone 27H11) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-1773CT | Recombinant Mouse anti-Mouse TNFSF11 Monoclonal antibody (23B779) | IHC-FoFr, WB | |
| HPAB-0685-CN | Human Anti-TNFSF11 Recombinant Antibody (HPAB-0685-CN) | Neut | Human IgG |
| HPAB-AP830-YC | Mouse Anti-TNFSF11 Recombinant Antibody (clone FLG5) | Inhib, FuncS | Mouse IgG |
| VS-0224-XY128 | Mouse Anti-TNFSF11 Recombinant Antibody (VS-0224-XY128) | ELISA, FC, IHC-P | Mouse IgG2b |
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