Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) (CAT#: TAB-334CQ)

This product is a recombinant mouse antibody that recognizes TL1A (Tumor necrosis factor-like ligand 1A). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.


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SDS-PAGE

Figure 1 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in SDS-PAGE

Figure 1 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in SDS-PAGE

SDS-PAGE analysis of TAB-334CQ in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of TAB-334CQ was greater than 95% as determined by SDS-PAGE.

SEC-HPLC

Figure 2 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in HPLC

Figure 2 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in HPLC

The purity of TAB-334CQ was 99.0172% as determined by SEC-HPLC.

WB

Figure 3 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in WB

Figure 3 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in WB

Western blot analysis of TAB-334CQ was performed by loading recombinant human TNFSF15 protein.

TAB-334CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-Mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 4 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in ELISA

Figure 4 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in ELISA

ELISA analysis of TAB-334CQ was performed by coating with recombinant human TNFSF15 protein. Then blocked with BSA, and incubated with Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ). The Goat Anti-Mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Immunogen
  • Human recombinant TL1A
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a, κ
  • Specificity
  • Human TL1A (Tumor necrosis factor-like ligand 1A)
  • Species Reactivity
  • Human
  • Applications
  • ELISA
  • Related Disease
  • Asthma, inflammatory bowel disease

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • TNFSF15; Tumor necrosis factor superfamily member 15; TL1; TL1A; VEGI; VEGI192A; tumor necrosis factor ligand superfamily member 15; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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