Rabbit Anti-TRIM28 Recombinant Antibody (clone DS3681AB)
CAT#: MOR-3681
Recombinant Rabbit anti-TRIM28 monoclonal antibody for WB, IHC-P, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/1132_B11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10066/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_9_7_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_5.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130726-TRIM28
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_4_6.jpg
Normal Tissue
Figure 9 Skin
Fibroblasts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_1.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
❮
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Specifications
- Immunogen
- Phospho-peptide corresponding to residues surrounding Serine 473 of Human KAP1.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human TRIM28
- Species Reactivity
- Human
- Clone
- DS3681AB
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "TRIM28"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-622 | Human Anti-TRIM28 Recombinant Antibody (clone D530) | ELISA, IP-MS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-054 | Recombinant Anti-human TRIM28 VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2165MZ | Recombinant Mouse Anti-Human TRIM28 Antibody | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0892-CN | Rabbit Anti-TRIM28 Recombinant Antibody (clone CBACN-336) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2242-CN | Rabbit Anti-TRIM28 Polyclonal Antibody (MRO-2242-CN) | WB, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1166 | Mouse Anti-TRIM28 Recombinant Antibody (clone 4E1-D12-F8) | WB, ICC, IHC, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1372 | AbPlus™ Anti-TRIM28 Magnetic Beads (VS-0724-YC1372) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7525 | Anti-TRIM28 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7526 | Anti-Mouse TRIM28 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7527 | Anti-Bovine TRIM28 Immunohistochemistry Kit | IHC |
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