Recombinant Mouse Antibody (D2. 3) is capable of binding to TSA, expressed in Chinese Hamster Ovary cells (CHO).
Figure 1 Hydrolysis of NP-ester 2p catalysed by antibodies D2.3 and D2.4.
(a) The time-course of p-nitrophenol (4p) release following the addition of NP-ester 2p (0.25 mM) to antibody D2.3 (0.35 μM) or D2.4 (0.1 μM). Inset, Rate of hydrolysis (v = dP/st) as a function of time (derived from equation (1); see Materials and Methods); the initial and steady-state rates od D2.3 (vᵢ and vf, respectively) are noted. (b) The steady-state rate, vf, and the lag rate constant, klag, exhibited by antibody D2.3 (1.5 μM) at different concentrations of ester 2p. The steady-state rate (vf) and concentration of substrate 2p (S₀) were fitted to the Michaelis-Menten model giving a KM and vfmax values of 25 μM and 7.8 μM min⁻¹, respectively.
Lindner, A. B., Eshhar, Z., & Tawfik, D. S. (1999). Conformational changes affect binding and catalysis by ester-hydrolysing antibodies1. Journal of molecular biology, 285(1), 421-430.
Figure 2 Binding affinities of ligands and their effect on pre-equilibrium of antibody D2.3.
Lindner, A. B., Eshhar, Z., & Tawfik, D. S. (1999). Conformational changes affect binding and catalysis by ester-hydrolysing antibodies1. Journal of molecular biology, 285(1), 421-430.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1009 | Recombinant Anti-TSA Antibody | WB, Dot, FuncS | IgG |
MHH-1009 | Recombinant Human Anti-TSA Antibody | WB, FC, FuncS | IgG |
PABL-351 | Mouse Anti-TSA Recombinant Antibody (clone 1D4) | IB, IP | Mouse IgG |
PABL-352 | Mouse Anti-TSA Recombinant Antibody (clone 6D9) | WB, ELISA, FuncS | Mouse IgG |
PABW-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.4) | ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1009-F(E) | Recombinant Anti-TSA Antibody Fab Fragment | IF, Neut, FuncS | Fab |
MHH-1009-F(E) | Recombinant Human Anti-TSA Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
PFBL-351 | Mouse Anti-TSA Recombinant Antibody (clone 1D4); Fab Fragment | WB, ELISA, IF, FuncS | Mouse Fab |
PFBL-352 | Mouse Anti-TSA Recombinant Antibody (clone 6D9); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
PFBZ-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.3); Fab Fragment | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1009-S(P) | Recombinant Anti-TSA Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
MHH-1009-S(P) | Recombinant Human Anti-TSA Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
PSBZ-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.3); scFv Fragment | ELISA | Mouse scFv |
PSBW-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.4); scFv Fragment | ELISA | Mouse scFv |
PSBC-126 | Mouse Anti-TSA Recombinant Antibody (clone D2.5); scFv Fragment | ELISA | Mouse scFv |
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