Mouse Anti-TTR Recombinant Antibody (clone 39-44) (CAT#: PABZ-128)

Figure 4 Immunoreactivity of size variants of the TTRs53–55 mutant separated by size-exclusion HPLC.

Size-exclusion HPLC of TTRs53–55 mutant freshly prepared (A) or aggregated in 37°C for 24 h (B). Immunoblot from native PAGE of size-exclusion HPLC fractions with polyclonal antibody (C), mAb 56–61 (D), or mAb 39–44 (E). Lanes 1–4 and 6–8 correspond to HPLC fractions as indicated; lane 5 was loaded with wild-type TTR. Lanes: 1–4, fresh material; 6–8, aggregated TTR. Arrows indicate the position of TTR tetramer; OVA (ovalbumin) and BSA indicate size markers.

Goldsteins, G., Persson, H., Andersson, K., Olofsson, A., Dacklin, I., Edvinsson, Å., ... & Lundgren, E. (1999). Exposure of cryptic epitopes on transthyretin only in amyloid and in amyloidogenic mutants. Proceedings of the National Academy of Sciences, 96(6), 3108-3113.

Figure 5 SAP co-localizes with TTR-A in Drosophila eye and counteracts TTR-induced retinal degeneration.

(A–D) TTR-A was detected with a TTR-specific monoclonal antibody (Mab39–44; in red), and co-localized with SAP immunostaining (Epitomics; in green) in horizontal sections of heads of 2-week-old flies. (E–H) TTR-A was detected with TTR-specific polyclonal antibody (red). TTR-A aggregates were monitored with p-FTAA (green). (A, B) TTR-A secreted by the photoreceptors accumulated in the retinal compartment (E, F) and formed aggregates around the outer corneal layer (CL). This led to damage of the retinal array and leakage of TTR-A outside the CL. The two neighboring corneal lenses (arrows) are shown magnified at the upper left corner (insets). (C) SAP expressed alone in fly retina stayed soluble, as no p-FTAA aggregates were detected (G) and there were no degenerative changes. Co-localization of SAP with TTR-A prevented retinal damage in SAP/TTR-A fruit flies (D), and led to reduced p-FTAA staining in the CL (H). Drosophila genotypes: TTR-A/− (w; GMR-Gal4/+; UAS-TTR-A/+); TTR-A/TTR-A (w; GMR-Gal4/GMR-Gal4; UAS-TTR-A/UAS-TTR-A); −/SAP (w; GMR-Gal4/+; +/UAS-SAP); TTR-A/SAP (w; GMR-Gal4/+; UAS-SAP/TTR-A). Scale bar represents 50 µm.

Andersson, K., Pokrzywa, M., Dacklin, I., & Lundgren, E. (2013). Inhibition of TTR Aggregation-Induced Cell Death–A New Role for Serum Amyloid P Component. PLoS One, 8(2), e55766.