Hamster Anti-Tcrb Recombinant Antibody (clone H57); Fab Fragment (CAT#: PFBC-090)

1. Q 1: Can the Fab fragment from clone H57 be used for in vivo studies involving T-cell receptor signaling?

   A: Yes, the Fab fragment of clone H57 is useful for in vivo studies as it binds specifically to Tcrb without inducing T-cell activation. It's particularly effective for blocking TCR signaling pathways.

2. Q 2: How does the usage of a Fab fragment differ from a full IgG antibody in terms of steric hindrance?

   A: Fab fragments, being smaller in size, can access epitopes that are sterically hindering. This results in more precise binding and decreases the chances of cross-linking and non-specific interactions.

3. Q 3: How should the antibody Fab fragment be prepared for use in immunoprecipitation assays?

   A: The Fab fragment for immunoprecipitation (IP) experiments needs to be pre-incubated with the cell lysate for at least an hour at 4°C. For effective IP, this should be followed by an incubation with protein A or G beads.

View the frequently asked questions answered by Creative Biolabs Support.