Anti-TrkA antibody is a Mouse of scFv class that binds to an TrkA. This antibody recognizes with high affinity and specificity the neurotrophin receptor TrkA and displays a neutralizing activity toward the NGF/TrkA interaction. This mouse monoclonal antibody, besides being highly specific and selective for the extracellular domain of human TrkA receptor, does not bind to any other closely related members of the Trk receptor super family.
Figure 1 Functional Assay OF MNAC13
Antiallodynic effects measured with a dynamic plantar aesthesiometer after four i.p. injections of 50 μg per mouse MNAC13 (from day 3 to day 6 after ligation of the sciatic nerve) in CCI mice tested for 14 days (IgG, n = 7; MNAC13, 50 μg per mouse, n = 5) (A) and after eight i.p. injections of 30 or 70 μg per mouse MNAC13 (from day 3 to day 10 after ligation of the sciatic nerve) in CCI mice tested from day 3 up to day 31 (B). (IgG, n = 11; MNAC13, 30 μg per mouse, n = 8; MNAC13, 70 μg per mouse, n = 12.) Black arrows represent the antibody i.p. administrations. BL (baseline) refers to mechanical threshold before operation. Black lines represent mechanical thresholds of sham-operated controls (n = 6). Data are presented as means ± SEM. *, P<0.05; **, P<0.01 vs. IgG-injected mice (Tukey/Kramer).
Ugolini, G., Marinelli, S., Covaceuszach, S., Cattaneo, A., & Pavone, F. (2007). The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain. Proceedings of the National Academy of Sciences, 104(8), 2985-2990.
Figure 2 The result of a FACS analysis on 3T3 TrkA+ cells
MNAC13 recognises the TrkA receptor on living cells. Balb/C 3T3 or TrkA+ Balb/C 3T3 cells were incubated with MNAC13 antibody and subjected to FACS analysis.
Figure 3 SPR analysis of MNAC13 binding to TrkA and p75 receptor
MNAC13 does not bind to the p75 receptor: Surface Plasmon Resonance experiments were performed by using a BIACore instrument (BIACore, Uppsala, Sweden).
Ugolini, G., Marinelli, S., Covaceuszach, S., Cattaneo, A., & Pavone, F. (2007). The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain. Proceedings of the National Academy of Sciences, 104(8), 2985-2990.
Figure 4 Phosphorylation Assay of TrkA affected by MNAC13
Western blot results showing that MNAC13 leads to reduction of phospho-TrkA levels after NGF stimulation in mouse 3T3 cells overexpressing TrkA. Antibodies were used at two different concentrations: 300 μg/ml (h) and 200 μg/ml (l).
Ugolini, G., Marinelli, S., Covaceuszach, S., Cattaneo, A., & Pavone, F. (2007). The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain. Proceedings of the National Academy of Sciences, 104(8), 2985-2990.
Figure 5 Inhibition of MNAC13
Western blot results showing that MNAC13 leads to inhibition of NGF-dependent phosphorylation of the PLCγ1 adaptor protein in mouse 3T3 cells overexpressing TrkA. Antibodies were used at two different concentrations: 300 μg/ml (h) and 200 μg/ml (l).
Ugolini, G., Marinelli, S., Covaceuszach, S., Cattaneo, A., & Pavone, F. (2007). The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain. Proceedings of the National Academy of Sciences, 104(8), 2985-2990.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1229z | Mouse Anti-NTRK1 Recombinant Antibody (clone 18B6) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
MOB-0841MZ | Recombinant Mouse Anti-Human NTRK1 Antibody (clone 7C3) | ELISA, IHC-P, WB | Mouse antibody |
HPAB-0659-YJ | Mouse Anti-NTRK1 Recombinant Antibody (clone 5C3) | Agonist, IHC, IF, Inhib | Mouse IgG2a, κ |
HPAB-0660-YJ | Human Anti-NTRK1 Recombinant Antibody (clone ch5C3) | Agonist, IHC, IF, Inhib | Chimeric (mouse/human) IgG1, κ |
VS3-CJ367 | Rabbit Anti-NTRK1 Recombinant Antibody (VS3-CJ367) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-170CQ | Human Anti-Human NTRK1 Recombinant Antibody (CRB0089) | ELISA, FC, WB | Human antibody |
TAB-170CQ-S(P) | Human Anti-Human NTRK1 Recombinant Antibody scFv Fragment (CRB0089) | ELISA, FC, WB | Human antibody |
TAB-170CQ-F(E) | Human Anti-Human NTRK1 Recombinant Antibody Fab Fragment (CRB0089) | ELISA, FC, WB | Human antibody |
TAB-511MZ | Anti-Human NTRK1 Recombinant Antibody (CRB0089) | ELISA, WB, FC | Human antibody |
TAB-511MZ-F(E) | Anti-Human NTRK1 Recombinant Antibody Fab Fragment (CRB0089) | ELISA, WB, FC | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0759MZ | Chicken Anti-TrkA Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-182 | Recombinant Mouse Anti-TrkA Antibody (MNAC13) | Neut, FuncS | IgG |
PABX-182-F (E) | Recombinant Mouse Anti-TrkA Antibody Fab Fragment (MNAC13) | Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2508 | Hi-Affi™ Recombinant Rabbit Anti-NTRK1 Monoclonal Antibody (DS2508AB) | WB, IHC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0396-CN-S(P) | Human Anti-NTRK1 Recombinant Antibody (clone GBR-VH5(V37A)VL1); scFv Fragment | ELISA, Block | Human scFv |
HPAB-0397-CN-S(P) | Human Anti-NTRK1 Recombinant Antibody (clone GBR-VH5(V37A)VL1(*)); scFv Fragment | ELISA, Block | Human scFv |
HPAB-AP400-YC-S(P) | Human Anti-NTRK1 Recombinant Antibody (clone CRB0036); scFv Fragment | ELISA, FC, WB, Inhib | Human scFv |
HPAB-AP401-YC-S(P) | Human Anti-NTRK1 Recombinant Antibody (clone CRB0069); scFv Fragment | ELISA, FC, WB, Inhib | Human scFv |
HPAB-0659-YJ-S(P) | Mouse Anti-NTRK1 Recombinant Antibody (clone 5C3); scFv Fragment | Agonist, IHC, IF, Inhib | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0396-CN-F(E) | Human Anti-NTRK1 Recombinant Antibody (clone GBR-VH5(V37A)VL1); Fab Fragment | ELISA, Block | Human Fab |
HPAB-0728-CN-F(E) | Mouse Anti-NTRK1 Recombinant Antibody (clone CRB0022); Fab Fragment | WB, ELISA, FC | Mouse Fab |
HPAB-0729-CN-F(E) | Human Anti-NTRK1 Recombinant Antibody (clone CRB0088); Fab Fragment | WB, ELISA, FC | Human Fab |
HPAB-0730-CN-F(E) | Human Anti-NTRK1 Recombinant Antibody (clone CRB0089_IgG4); Fab Fragment | WB, ELISA, FC | Human Fab |
HPAB-0660-YJ-F(E) | Human Anti-NTRK1 Recombinant Antibody (clone ch5C3); Fab Fragment | Agonist, IHC, IF, Inhib | Chimeric (mouse/human) Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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