Anti-Human UPAR Recombinant Antibody (19.1) (CAT#: TAB-0395CL)

Figure 1 Inhibition of uPAR-dependent adhesion of HEK293 cells on vitronectin by mAbs from epitope bin 6.

The inhibitory effects of anti-uPAR mAbs (100 nM) belonging to epitope bin 6 (mAbs 8B12 and 19.10) and bin 1 (mAb R21) were compared on adherent HEK293 cells. The HEK293 cells were induced to adhere firmly on vitronectin by their surface expression of uPARwt, uPARwt·ATF complexes (formed by addition of 50 nM ATF), or uPARH47C-N259C. Cellular protrusions were scored 24 h after addition of the respective mAbs or buffer controls and each bar represents the mean value of three independent measurements (a protrusion index of 100% signifies that all 18 micrographs examined were scored positive for the presence of lamellipodia by both evaluators). Note the that the y-axis has been shifted upwards to allow the visualization of zero values.

Mandik-Nayak, L., DuHadaway, J. B., Mulgrew, J., Pigott, E., Manley, K., Sedano, S., ... & Laury-Kleintop, L. D. (2017). RhoB blockade selectively inhibits autoantibody production in autoimmune models of rheumatoid arthritis and lupus. Disease models & mechanisms, 10(11), 1313-1322.