Human Anti-VEGFA Recombinant Antibody (clone 5A12) (CAT#: PABL-358)
Recombinant Human Antibody (5A12) is capable of binding to VEGF, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-VEGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-VEGF mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
BL
Figure 1 Affinity improved variants with sub-nanomolar dual affinity show similar blocking activity as mono-specific antibodies.
In vitro receptor blocking assay to compare the blocking of hAng2 binding to Tie2 by 5A12, the high affinity 5A12 variants, and the mono-specific hAng2 and hVEGF antibody G5.5 and G6.31, respectively.
Koenig, P., Lee, C. V., Sanowar, S., Wu, P., Stinson, J., Harris, S. F., & Fuh, G. (2015). Deep sequencing-guided design of a high affinity dual specificity antibody to target two angiogenic factors in neovascular age-related macular degeneration. Journal of Biological Chemistry, 290(36), 21773-21786.
Specifications
- Immunogen
- Human vascular endothelial growth factor A
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human VEGFA
- Species Reactivity
- Human
- Clone
- 5A12
- Applications
- Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Blocking.
Target
- Alternative Names
- VEGFA; vascular endothelial growth factor A; VPF; VEGF; MVCD1; vascular permeability factor
- Gene ID
- 7422
- UniProt ID
- P15692
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H73 | Anti-Human VEGFA Recombinant Antibody (Vanucizumab) | WB, FC, IP, ELISA, Neut, FuncS, IF | VH - C-kappa - CH2 - CH3 _ V-lambda - CH1 _ H-GAMMA-1 _ L-kappa |
| TAB-307CQ | Human Anti-VEGFA Recombinant Antibody (TAB-307CQ) | Inhib | Human IgG |
| TAB-307CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-307CQ-S(P)) | ELISA, Neut | Human scFv |
| TAB-307CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-307CQ-F(E)) | ELISA, Neut | Humanized Fab |
| TAB-009ML | Anti-Human VEGFA Recombinant Antibody scFv Fragment (Brolucizumab) | ELISA, IHC, FC, IP, IF, Inhib | scFv, κ |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L043L | anti-VEGFA immunotoxin Ng76 (scFv)-Luffin-β | Cytotoxicity assay, Functional assay |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-284CQ | Human Anti-VEGFA Recombinant Antibody (TAB-284CQ) | WB | Human antibody |
| TAB-284CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-284CQ-F(E)) | ELISA | Human Fab |
| TAB-304CQ-F(E) | Anti-Human VEGFA Recombinant Antibody Fab Fragment (2H1) | ELISA, WB | Human antibody |
| TAB-305CQ-F(E) | Anti-Human VEGFA Recombinant Antibody Fab Fragment (2H1-32) | ELISA, WB | Human antibody |
| TAB-465CQ | Anti-Human VEGFA Recombinant Antibody (Varisacumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-306CQ | Mouse Anti-VEGFA Recombinant Antibody (TAB-306CQ) | FuncS, Inhib | Mouse IgG |
| TAB-306CQ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-306CQ-S(P)) | ELISA, Neut | Mouse scFv |
| TAB-306CQ-F(E) | Mouse Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-306CQ-F(E)) | ELISA, Neut | Mouse Fab |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0623MZ | Chicken Anti-VEGF Polyclonal IgY | WB | Chicken antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2255CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL198) | Neut, WB | Mouse IgG1 |
| NEUT-2256CQ | Rat Anti-Vegfa Recombinant Antibody (clone 2G11-2A05) | WB, Neut, ELISA, IHC, FuncS | Rat IgG2a, κ |
| NEUT-2257CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 16F1) | WB, Neut | Mouse IgG2a |
| NEUT-2258CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 4B2-8) | ELISA, Neut, WB | Mouse IgG1, κ |
| NEUT-2260CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL737) | WB, ELISA, Neut | Mouse IgG2b |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2259CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A.4.6.1) | ELISA, IP, WB, Block, FC, IHC | Mouse IgG1, κ |
| NEUT-2267CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A15136B) | WB, Block | Mouse IgG2b, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3835 | Rabbit Anti-VEGFA Recombinant Antibody (clone DS3835AB) | FC, ICC, IF, IHC-P, IP, WB | Rabbit IgG |
| MOR-4758 | Rabbit Anti-Vegfa Recombinant Antibody (clone TH272DS) | WB | Rabbit IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MD-ZBF-YC024 | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone A80887) | ELlSA | Camelid VHH |
| HPAB-0337-YJ-vNAR | Recombinant Shark Anti-VEGFA vNAR (V32R) | FC, ELISA, Inhib, Neut | Shark vNAR |
| HPAB-0338-YJ-vNAR | Recombinant Shark Anti-VEGFA vNAR (V19) | FC, ELISA, Inhib, Neut | Shark vNAR |
| HPAB-0774-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (CBV73YJ) | ELISA | Camelid VHH |
| NBD-0090-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (Nb42) | ELISA, WB, Inhib | Camelid VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0151-FY-F(E) | Human Anti-VEGFA Recombinant Antibody (clone rhVVH); Fab Fragment | WB, ELISA, Inhib | Human Fab |
| HPAB-0348-WJ-F(E) | Human Anti-VEGFA Recombinant Antibody (clone 7F2); Fab Fragment | ELISA | Humanized Fab |
| HPAB-N0295-YC-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (HPAB-N0295-YC-F(E)) | ELISA | Human Fab |
| HPAB-N0296-YC-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (HPAB-N0296-YC-F(E)) | ELISA | Human Fab |
| HPAB-0336-YJ-F(E) | Human Anti-VEGFA Recombinant Antibody (clone BAT-1706); Fab Fragment | FuncS | Humanized Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0004-WJ | Human Anti-VEGFA Recombinant Antibody (clone 8B13) | ELISA | Humanized IgG |
| MRO-2269-CN | Rabbit Anti-VEGFA Polyclonal Antibody (MRO-2269-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0243-YJ | Mouse Anti-VEGFA Recombinant Antibody (clone 16-3) | ELISA, Block | Mouse IgG1,κ |
| HPAB-0244-YJ | Mouse Anti-VEGFA Recombinant Antibody (clone 16-5) | ELISA, Block | Mouse IgG2b,κ |
| HPAB-M0372-YC | Canine Anti-VEGFA Recombinant Antibody (HPAB-M0372-YC) | FuncS, Inhib | Chimeric (Mouse/Canine) IgGB, κ |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-465CQ | Afuco™ Anti-VEGFA ADCC Recombinant Antibody (Varisacumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-011 | Afuco™ Anti-VEGFA ADCC Recombinant Antibody (Bevacizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1245WJ-S(P) | Human Anti-VEGFA Recombinant Antibody (clone AM2); scFv Fragment | ELISA | Human scFv |
| HPAB-1956-FY-S(P) | Human Anti-VEGFA Recombinant Antibody (clone Bh21-7); scFv Fragment | IF | Humanized scFv |
| HPAB-2045-FY-S(P) | Human Anti-VEGFA Recombinant Antibody (clone C1094); scFv Fragment | IA | Human scFv |
| HPAB-2046-FY-S(P) | Human Anti-VEGFA Recombinant Antibody (clone C1039); scFv Fragment | IA | Human scFv |
| HPAB-0677YY-S(P) | Mouse Anti-VEGFA Recombinant Antibody (clone CBV110YJ); scFv Fragment | ELISA, FuncS | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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