Human Anti-VEGFA Recombinant Antibody (clone bH1) (CAT#: PABZ-131)
Recombinant Human Antibody (bH1) is capable of binding to VEGFA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-VEGFA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti- VEGFA mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody inhibits both HER2- and VEGF-mediated cell proliferation in vitro and tumor progression in mouse models.
Specific Inquiry
Inhib
Figure 1 Inhibition of VEGF and HER2 function in vitro and in vivo.
bH1 and the affinity improved variants bH1-81 and bH1-44 as IgGs inhibit VEGF-stimulated proliferation of HUVECs in a dose-dependent manner. Herceptin and bevacizumab (anti-VEGF) were used as controls. Error bars represent the average deviation of triplicates (n = 3).
Bostrom, J., Yu, S. F., Kan, D., Appleton, B. A., Lee, C. V., Billeci, K., ... & Fuh, G. (2009). Variants of the antibody herceptin that interact with HER2 and VEGF at the antigen binding site. Science, 323(5921), 1610-1614.
Specifications
- Immunogen
- Human vascular endothelial growth factor A
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human VEGFA
- Species Reactivity
- Human
- Clone
- bH1
- Applications
- Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibition.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H73 | Anti-Human VEGFA Recombinant Antibody (Vanucizumab) | WB, FC, IP, ELISA, Neut, FuncS, IF | VH - C-kappa - CH2 - CH3 _ V-lambda - CH1 _ H-GAMMA-1 _ L-kappa |
| TAB-011 | Anti-Human VEGF Recombinant Antibody (Bevacizumab) | FC, IP, ELISA, Neut, FuncS, IF, ICC | IgG1 - kappa |
| TAB-012-F(E) | Anti-Human VEGF Recombinant Antibody Fab Fragment (Ranibizumab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Fab - G1 - kappa |
| TAB-307CQ | Human Anti-VEGFA Recombinant Antibody (TAB-307CQ) | Inhib | Human IgG |
| TAB-307CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-307CQ-S(P)) | ELISA, Neut | Human scFv |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L043L | anti-VEGFA immunotoxin Ng76 (scFv)-Luffin-β | Cytotoxicity assay, Functional assay |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-075 | Mouse Anti-VEGFA Recombinant Antibody (clone YADS1) | ELISA | Mouse IgG |
| PABC-076 | Mouse Anti-VEGFA Recombinant Antibody (clone YADS2) | ELISA | Mouse IgG |
| FAMAB-0331-CN-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone VA12) | WB, ELISA | Camelid VHH |
| HPAB-0151-FY | Human Anti-VEGFA Recombinant Antibody (clone rhVVH) | WB, ELISA, Inhib | Human IgM |
| HPAB-0004-WJ | Human Anti-VEGFA Recombinant Antibody (clone 8B13) | ELISA | Humanized IgG |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-284CQ | Human Anti-VEGFA Recombinant Antibody (TAB-284CQ) | WB | Human antibody |
| TAB-284CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-284CQ-S(P)) | ELISA | Human scFv |
| TAB-304CQ-S(P) | Anti-Human VEGFA Recombinant Antibody scFv Fragment (2H1) | ELISA, WB | Human antibody |
| TAB-305CQ-S(P) | Anti-Human VEGFA Recombinant Antibody scFv Fragment (2H1-32) | ELISA, WB | Human antibody |
| TAB-284CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-284CQ-F(E)) | ELISA | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-306CQ | Mouse Anti-VEGFA Recombinant Antibody (TAB-306CQ) | FuncS, Inhib | Mouse IgG |
| TAB-306CQ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-306CQ-S(P)) | ELISA, Neut | Mouse scFv |
| TAB-306CQ-F(E) | Mouse Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-306CQ-F(E)) | ELISA, Neut | Mouse Fab |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0623MZ | Chicken Anti-VEGF Polyclonal IgY | WB | Chicken antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2255CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL198) | Neut, WB | Mouse IgG1 |
| NEUT-2256CQ | Rat Anti-Vegfa Recombinant Antibody (clone 2G11-2A05) | WB, Neut, ELISA, IHC, FuncS | Rat IgG2a, κ |
| NEUT-2257CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 16F1) | WB, Neut | Mouse IgG2a |
| NEUT-2258CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 4B2-8) | ELISA, Neut, WB | Mouse IgG1, κ |
| NEUT-2260CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL737) | WB, ELISA, Neut | Mouse IgG2b |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2259CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A.4.6.1) | ELISA, IP, WB, Block, FC, IHC | Mouse IgG1, κ |
| NEUT-2267CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A15136B) | WB, Block | Mouse IgG2b, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3835 | Rabbit Anti-VEGFA Recombinant Antibody (clone DS3835AB) | FC, ICC, IF, IHC-P, IP, WB | Rabbit IgG |
| MOR-4758 | Rabbit Anti-Vegfa Recombinant Antibody (clone TH272DS) | WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0330CQ-F(E) | Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31); Fab Fragment | ELISA, IF, Block | Chimeric (mouse/human) Fab |
| HPAB-0243-YJ-F(E) | Mouse Anti-VEGFA Recombinant Antibody (clone 16-3); Fab Fragment | ELISA, Block | Mouse Fab |
| HPAB-0244-YJ-F(E) | Mouse Anti-VEGFA Recombinant Antibody (clone 16-5); Fab Fragment | ELISA, Block | Mouse Fab |
| HPAB-0348-WJ-F(E) | Human Anti-VEGFA Recombinant Antibody (clone 7F2); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0349-WJ-F(E) | Human Anti-VEGFA Recombinant Antibody (clone 6B2); Fab Fragment | ELISA | Humanized Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-465CQ | Afuco™ Anti-VEGFA ADCC Recombinant Antibody (Varisacumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-011 | Afuco™ Anti-VEGFA ADCC Recombinant Antibody (Bevacizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0310-YC-VHNAR | Recombinant Shark Anti-VEGFA Single Domain Antibody (V32R) | ELISA, FC, WB | Shark vNAR |
| HPAB-N0311-YC-VHNAR | Recombinant Shark Anti-VEGFA Single Domain Antibody (V19) | ELISA, FC, WB | Shark vNAR |
| HPAB-0646-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone VEvhh1) | ELISA, Inhib | Camelid VHH |
| HPAB-0647-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone VEvhh2) | ELISA, Inhib | Camelid VHH |
| HPAB-0648-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone VEvhh3) | ELISA, Inhib | Camelid VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0114-WJ-S(P) | Human Anti-VEGFA Recombinant Antibody (clone FD006); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0152-WJ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (HPAB-0152-WJ-S(P)) | ELISA | Human scFv |
| HPAB-0922-WJ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (HPAB-0922-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-1245WJ-S(P) | Human Anti-VEGFA Recombinant Antibody (clone AM2); scFv Fragment | ELISA | Human scFv |
| HPAB-0677YY-S(P) | Mouse Anti-VEGFA Recombinant Antibody (clone CBV110YJ); scFv Fragment | ELISA, FuncS | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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