Anti-Human VEGFA Recombinant Antibody (TAB-H73)
CAT#: TAB-H73
Recombinant Humanized antibody to Human VEGFA
Gene Expression
Tested Data
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA
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SDS-PAGE
Figure 1 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in SDS-PAGE
SDS-PAGE analysis of TAB-H73 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 48-63 kDa and 25 kDa, respectively.
WB
Figure 2 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in WB
Western blot analysis of TAB-H73 was performed by loading Human VEGF165 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H73 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
WB
Figure 3 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in WB
Western blot analysis of TAB-H73 was performed by loading Human VEGF121 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H73 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
DB
Figure 4 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in DB
Dot Blot analysis of TAB-H73 was performed by coating with Human VEGF165 Protein (His Tag).
TAB-H73 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
DB
Figure 5 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in DB
Dot Blot analysis of TAB-H73 was performed by coating with Human VEGF121 Protein (His Tag).
TAB-H73 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 6 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in ELISA
ELISA analysis of TAB-H73 was performed by coating with Human VEGF165 Protein (His Tag). Then blocked with BSA, and incubated with TAB-H73. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
ELISA
Figure 7 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in ELISA
ELISA analysis of TAB-H73 was performed by coating with Human VEGF121 Protein (His Tag). Then blocked with BSA, and incubated with TAB-H73. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- undefined
- Derivation
- Humanized
- Type
- VH - C-kappa - CH2 - CH3 _ V-lambda - CH1 _ H-GAMMA-1 _ L-kappa
- Specificity
- VEGFA (vascular endothelial growth factor A, VEGF-A, VEGF) [Homo sapiens] ;
- Species Reactivity
- Human
- Applications
- WB, FC, IP, ELISA, Neut, FuncS, IF
- Related Disease
- Solid tumors
Product Property
- Purity
- >95.0% as determined by analysis by RP-HPLC.
Applications
- Application Notes
- The VEGFA antibody has been reported in applications of MRI, IHC.
MRI: Colo205 colon cancer xenografts were imaged before and 5 days after treatment with a single 10 mg kg−1 dose of either vanucizumab, bevacizumab (anti-human VEGF-A), LC06 (anti-murine/human Ang-2) or omalizumab (anti-human IgE control). Volumetric response was assessed using T2-weighted MRI, and diffusion-weighted, dynamic contrast-enhanced (DCE) and susceptibility contrast MRI used to quantify tumour water diffusivity (apparent diffusion coefficient (ADC), × 106 mm2 s−1), vascular perfusion/permeability (Ktrans, min−1) and fractional blood volume (fBV, %) respectively. Pathological correlates were sought, and preliminary gene expression profiling performed.
Target
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Biosimilar Overview
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-011 | Anti-Human VEGF Recombinant Antibody (Bevacizumab) | FC, IP, ELISA, Neut, FuncS, IF, ICC | IgG1 - kappa |
| TAB-012-F(E) | Anti-Human VEGF Recombinant Antibody Fab Fragment (TAB-012-F(E)) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Fab - G1 - kappa |
| TAB-307CQ | Human Anti-VEGFA Recombinant Antibody (TAB-307CQ) | Inhib | Human IgG |
| TAB-307CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-307CQ-S(P)) | ELISA, Neut | Human scFv |
| TAB-009ML | Anti-Human VEGFA Recombinant Antibody scFv Fragment (TAB-009ML) | ELISA, IHC, FC, IP, IF, Inhib | scFv, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L043L | anti-VEGFA immunotoxin Ng76 (scFv)-Luffin-β | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-075 | Mouse Anti-VEGFA Recombinant Antibody (clone YADS1); Fab Fragment | ELISA | Mouse Fab |
| PFBC-076 | Mouse Anti-VEGFA Recombinant Antibody (clone YADS2); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0004-WJ-F(E) | Human Anti-VEGFA Recombinant Antibody (clone 8B13); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0715-FY-S(P) | Mouse Anti-VEGFA Recombinant Antibody (clone AB014); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0950-FY-S(P) | Camelid Anti-VEGFA Recombinant Antibody; scFv Fragment (HPAB-0950-FY-S(P)) | Inhib | Camelid scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-131 | Human Anti-VEGFA Recombinant Antibody (clone bH1); scFv Fragment | WB, IF, FuncS | Human scFv |
| PSBW-131 | Human Anti-VEGFA Recombinant Antibody (clone YC159); scFv Fragment | WB, ELISA | Human scFv |
| HPAB-J0270-YC-S(P) | Human Anti-VEGFA Recombinant Antibody (clone XPA.10.064.03); scFv Fragment | ELISA, Inhib | Human scFv |
| HPAB-J0271-YC-S(P) | Human Anti-VEGFA Recombinant Antibody (clone XPA.10.064.04); scFv Fragment | ELISA, Inhib | Human scFv |
| HPAB-J0272-YC-S(P) | Human Anti-VEGFA Recombinant Antibody (clone XPA.10.064.06); scFv Fragment | ELISA, Inhib, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-284CQ | Human Anti-VEGFA Recombinant Antibody (TAB-284CQ) | WB | Human antibody |
| TAB-284CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-284CQ-S(P)) | ELISA | Human scFv |
| TAB-304CQ-S(P) | Anti-Human VEGFA Recombinant Antibody scFv Fragment (2H1) | ELISA, WB | Human antibody |
| TAB-305CQ-S(P) | Anti-Human VEGFA Recombinant Antibody scFv Fragment (2H1-32) | ELISA, WB | Human antibody |
| TAB-465CQ | Anti-Human VEGFA Recombinant Antibody (TAB-465CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-306CQ | Mouse Anti-VEGFA Recombinant Antibody (TAB-306CQ) | FuncS, Inhib | Mouse IgG |
| TAB-306CQ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-306CQ-S(P)) | ELISA, Neut | Mouse scFv |
| TAB-306CQ-F(E) | Mouse Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-306CQ-F(E)) | ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0623MZ | Chicken Anti-VEGF Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2258CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 4B2-8) | ELISA, Neut, WB | Mouse IgG1, κ |
| NEUT-2260CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL737) | WB, ELISA, Neut | Mouse IgG2b |
| NEUT-2261CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL738) | WB, IHC, ELISA, Neut | Mouse IgG2b |
| NEUT-2262CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL928) | Neut | Mouse IgG1, κ |
| NEUT-2263CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL984) | ELISA, Neut, WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2259CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A.4.6.1) | ELISA, IP, WB, Block, FC, IHC | Mouse IgG1, κ |
| NEUT-2267CQ | Mouse Anti-VEGFA Recombinant Antibody (NEUT-2267CQ) | WB, Block | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3835 | Rabbit Anti-VEGFA Recombinant Antibody (clone DS3835AB) | FC, ICC, IF, IHC-P, IP, WB | Rabbit IgG |
| MOR-4758 | Rabbit Anti-Vegfa Recombinant Antibody (clone TH272DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0151-FY | Human Anti-VEGFA Recombinant Antibody (clone rhVVH) | WB, ELISA, Inhib | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1578-CN | Rabbit Anti-VEGFA Recombinant Antibody (clone CBACN-578) | IF, IHC, IP, FC | Rabbit IgG |
| MRO-2269-CN | Rabbit Anti-VEGFA Polyclonal Antibody (MRO-2269-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-M0372-YC | Canine Anti-VEGFA Recombinant Antibody (HPAB-M0372-YC) | FuncS, Inhib | Chimeric (Mouse/Canine) IgGB, κ |
| HPAB-M0373-YC | Canine Anti-VEGFA Recombinant Antibody (HPAB-M0373-YC) | ELISA, Inhib, FuncS | Chimeric (Mouse/Canine) IgGB, κ |
| HPAB-M0374-YC | Canine Anti-VEGFA Recombinant Antibody (HPAB-M0374-YC) | ELISA, Inhib, FuncS | Chimeric (Mouse/Canine) IgGB, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-465CQ | Afuco™ Anti-VEGFA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-465CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-011 | Afuco™ Anti-VEGFA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-011) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0646-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (HPAB-0646-YJ-VHH) | ELISA, Inhib | Camelid VHH |
| HPAB-0647-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (HPAB-0647-YJ-VHH) | ELISA, Inhib | Camelid VHH |
| HPAB-0648-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (HPAB-0648-YJ-VHH) | ELISA, Inhib | Camelid VHH |
| HPAB-0656-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (HPAB-0656-YJ-VHH) | ELISA, WB, Inhib | Camelid VHH |
| HPAB-0657-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (HPAB-0657-YJ-VHH) | ELISA, WB, Inhib | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY273 | AbPlus™ Anti-VEGFA Magnetic Beads (A.4.6.1) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY540 | Mouse Anti-NHP VEGFA Recombinant Antibody (clone 26503) | WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY46 | Human Anti-VEGFA (clone G6-31) scFv-Fc Chimera | Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC709 | Recombinant Anti-VEGFA Vesicular Antibody, EV Displayed (VS-0425-YC709) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC190 | Recombinant Anti-VEGFA (N-terminus x C-terminus) Biparatopic Antibody, Tandem scFv (Clone C1039 x Clone c870) | ELISA | Tandem scFv |
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