anti-VEGFA immunotoxin Ng76 (scFv)-Luffin-β (CAT#: AGTO-L043L)
This immunotoxin was achieved by conjugating/fusing the Anti-VEGFA scFv to loofah toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to melanoma cells and the toxin enters cells by receptor-mediated endocytosis. Then loofah toxin inhibits protein synthesis by damaging the 60S subunit of mammalian ribosomes. It was designed for treatment of melanoma.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg
(Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg
Figure 2 Thyroid gland
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg
Figure 3 Colon
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg
(Cholangiocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg
(Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg
(Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000112715-VEGFA
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000112715-VEGFA
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- Ng76
- Molecule Class
- scFv-Luffin-β
- Toxin Description
- Loofah toxin (Luffin-β), is separated from the silk seeds into type I ribosome inactivating protein (ribosomeinactivating protein, RIP), RIP gourd. There are many types, mainly Luffin-a, Luffin-β, Luffin-Ss, Luffin-cylin, Luffin-Pl, which Luffin-β point containing 278 amino acids, the molecular weight of 30. 583KD, etc. 9.5, capable of specifically hydrolyzing eukaryotic ribosomes 28SrRNA4324 bit of adenine, so true 60s nuclear large subunit of ribosomes inactivate significantly inhibit protein synthesis, is one of the most active single-chain RIP are currently in, is of concern immunotoxin new powerful warhead small molecule recent years
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Melanoma
Target
- Alternative Names
- VPF; VEGF; MVCD1; VEGFA; vascular endothelial growth factor A; Melanoma; Loofah toxin; Loofah; Luffin-β; Luffin B; Luffin
- Gene ID
- 7422
- UniProt ID
- P15692
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-011 | Anti-Human VEGF Recombinant Antibody (Bevacizumab) | FC, IP, ELISA, Neut, FuncS, IF, ICC | IgG1 - kappa |
| TAB-012-F(E) | Anti-Human VEGF Recombinant Antibody Fab Fragment (Ranibizumab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Fab - G1 - kappa |
| TAB-307CQ | Human Anti-VEGFA Recombinant Antibody (TAB-307CQ) | Inhib | Human IgG |
| TAB-307CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-307CQ-S(P)) | ELISA, Neut | Human scFv |
| TAB-307CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-307CQ-F(E)) | ELISA, Neut | Humanized Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-284CQ | Human Anti-VEGFA Recombinant Antibody (TAB-284CQ) | WB | Human antibody |
| TAB-284CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-284CQ-F(E)) | ELISA | Human Fab |
| TAB-304CQ-F(E) | Anti-Human VEGFA Recombinant Antibody Fab Fragment (2H1) | ELISA, WB | Human antibody |
| TAB-305CQ-F(E) | Anti-Human VEGFA Recombinant Antibody Fab Fragment (2H1-32) | ELISA, WB | Human antibody |
| TAB-465CQ | Anti-Human VEGFA Recombinant Antibody (Varisacumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-306CQ | Mouse Anti-VEGFA Recombinant Antibody (TAB-306CQ) | FuncS, Inhib | Mouse IgG |
| TAB-306CQ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-306CQ-S(P)) | ELISA, Neut | Mouse scFv |
| TAB-306CQ-F(E) | Mouse Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-306CQ-F(E)) | ELISA, Neut | Mouse Fab |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0623MZ | Chicken Anti-VEGF Polyclonal IgY | WB | Chicken antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2255CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL198) | Neut, WB | Mouse IgG1 |
| NEUT-2256CQ | Rat Anti-Vegfa Recombinant Antibody (clone 2G11-2A05) | WB, Neut, ELISA, IHC, FuncS | Rat IgG2a, κ |
| NEUT-2257CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 16F1) | WB, Neut | Mouse IgG2a |
| NEUT-2258CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 4B2-8) | ELISA, Neut, WB | Mouse IgG1, κ |
| NEUT-2260CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL737) | WB, ELISA, Neut | Mouse IgG2b |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2259CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A.4.6.1) | ELISA, IP, WB, Block, FC, IHC | Mouse IgG1, κ |
| NEUT-2267CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A15136B) | WB, Block | Mouse IgG2b, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3835 | Rabbit Anti-VEGFA Recombinant Antibody (clone DS3835AB) | FC, ICC, IF, IHC-P, IP, WB | Rabbit IgG |
| MOR-4758 | Rabbit Anti-Vegfa Recombinant Antibody (clone TH272DS) | WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0151-FY-F(E) | Human Anti-VEGFA Recombinant Antibody (clone rhVVH); Fab Fragment | WB, ELISA, Inhib | Human Fab |
| HPAB-0243-YJ-F(E) | Mouse Anti-VEGFA Recombinant Antibody (clone 16-3); Fab Fragment | ELISA, Block | Mouse Fab |
| HPAB-0244-YJ-F(E) | Mouse Anti-VEGFA Recombinant Antibody (clone 16-5); Fab Fragment | ELISA, Block | Mouse Fab |
| HPAB-0348-WJ-F(E) | Human Anti-VEGFA Recombinant Antibody (clone 7F2); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0349-WJ-F(E) | Human Anti-VEGFA Recombinant Antibody (clone 6B2); Fab Fragment | ELISA | Humanized Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1578-CN | Rabbit Anti-VEGFA Recombinant Antibody (clone CBACN-578) | IF, IHC, IP, FC | Rabbit IgG |
| HPAB-0243-YJ | Mouse Anti-VEGFA Recombinant Antibody (clone 16-3) | ELISA, Block | Mouse IgG1,κ |
| HPAB-0244-YJ | Mouse Anti-VEGFA Recombinant Antibody (clone 16-5) | ELISA, Block | Mouse IgG2b,κ |
| HPAB-M0242-YC | Human Anti-VEGFA Recombinant Antibody (clone B5E3) | ELISA | Humanized IgG |
| HPAB-M0243-YC | Human Anti-VEGFA Recombinant Antibody (clone Bev-B5E3BsAb) | ELISA, Block, Inhib | Humanized IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-465CQ | Afuco™ Anti-VEGFA ADCC Recombinant Antibody (Varisacumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-011 | Afuco™ Anti-VEGFA ADCC Recombinant Antibody (Bevacizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0337-YJ-vNAR | Recombinant Shark Anti-VEGFA vNAR (V32R) | FC, ELISA, Inhib, Neut | Shark vNAR |
| HPAB-0338-YJ-vNAR | Recombinant Shark Anti-VEGFA vNAR (V19) | FC, ELISA, Inhib, Neut | Shark vNAR |
| HPAB-0646-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone VEvhh1) | ELISA, Inhib | Camelid VHH |
| HPAB-0647-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone VEvhh2) | ELISA, Inhib | Camelid VHH |
| HPAB-0648-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (clone VEvhh3) | ELISA, Inhib | Camelid VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0922-WJ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (HPAB-0922-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-1956-FY-S(P) | Human Anti-VEGFA Recombinant Antibody (clone Bh21-7); scFv Fragment | IF | Humanized scFv |
| HPAB-2044-FY-S(P) | Human Anti-VEGFA Recombinant Antibody (clone c870); scFv Fragment | IA | Human scFv |
| HPAB-2045-FY-S(P) | Human Anti-VEGFA Recombinant Antibody (clone C1094); scFv Fragment | IA | Human scFv |
| HPAB-2046-FY-S(P) | Human Anti-VEGFA Recombinant Antibody (clone C1039); scFv Fragment | IA | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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