Mouse Anti-VWF Recombinant Antibody (clone NMC-4) (CAT#: PABW-132)

• Immunogen
• Human Von Willebrand Factor

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human VWF

• Species Reactivity
• Human

• Clone
• NMC-4

• Applications
• Block, Inhib, WB, DB

• Purity
• >95% as determined by SDS-PAGE and HPLC analysis

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• The VWF antibody has been reported in applications of Blocking, Inhibition, Western Blot, Dot Blot.
  For Inhibiton: Formalin-ked platelets were prepared as previously described and used at a concentration of 10⁸ platelets/mL in an incubation volume of 125 pL. [¹²⁵I]vWF or [¹²⁵I]AS-vWF was added at a concentration of 5 or 20 µg/mL in the presence of competing ligands or MoAbs at concentrations described in Results. For vWF binding, ristocetin or botrocetin was used at a final concentration of 1 mg/mL or 15 µg/mL. Binding was measured after 30 minutes of incubation at room temperature, and nonspecific binding was determined in the presence of a 50-fold excess of unlabeledvWF or AS-vWF. Specific binding was calculated by subtracting nonspecific binding from the total.

• Alternative Names
• VWF; VWD; Von Willebrand Factor; Coagulation Factor VIII VWF; VWF

• Gene ID
• 7450

• UniProt ID
• P04275

• PFBW-132
• Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment

• PSBW-132
• Mouse Anti-VWF Recombinant Antibody (clone NMC-4); scFv Fragment

• PABW-132-mRNA
• Mouse Anti-VWF Antibody (clone NMC-4), mRNA

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