Rabbit Anti-ZAP70 Recombinant Antibody (clone TH230DS)
CAT#: MOR-4716
Recombinant Rabbit anti-ZAP70 monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_A_8_5.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_B_5_4.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_A_7_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115085-ZAP70/subcellular
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Specifications
- Immunogen
- A peptide corresponding to amino acids 311-323 of P43403.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ZAP70
- Species Reactivity
- Human
- Clone
- TH230DS
- Applications
- WB, IF, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- FC 1-3 µg/test
ICC 1-3 µg/mL
IF 1-3 µg/mL
WB 0.5-2 µg/mL
Target
- Alternative Names
- Zeta Chain Of T Cell Receptor Associated Protein Kinase 70kDa; Zeta-Chain (TCR) Associated Protein Kinase 70kDa; 70 KDa Zeta-Chain Associated Protein; Syk-Related Tyrosine Kinase; EC 2.7.10.2; SRK; Zeta-Chain (TCR) Associated Protein Kinase (70 KD); Zeta-Chain Associated Protein Kinase, 70kD; 70 KDa Zeta-Associated Protein; EC 2.7.10; ZAP-70; STCD; STD; TZK
- Gene ID
- 7535
- UniProt ID
- P43403
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Related Signaling Pathways
BCR Signaling Pathway
TCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ZAP70"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1794MZ | Recombinant Mouse Anti-Human ZAP70 Antibody (clone 22I50) | WB | Mouse antibody |
| MRO-1610-CN | Rabbit Anti-ZAP70 Recombinant Antibody (clone JU08-39) | WB, IF, IHC, FC, IP | Rabbit IgG |
| VS3-CJ962 | Mouse Anti-ZAP70 Recombinant Antibody (VS3-CJ962) | WB, IHC | Mouse IgG2b |
| VS7-0425-WR1146 | Mouse Anti-ZAP70 Monoclonal Antibody (clone 3D2A4) | WB, IHC | Mouse IgG1 |
| VS7-0425-WR1147 | Mouse Anti-ZAP70 Monoclonal Antibody (VS7-0425-WR1147) | WB, IHC-P, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3911 | Rabbit Anti-ZAP70 Recombinant Antibody (clone DS3911AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1237 | CytoStream™ Rabbit Anti-ZAP70 Recombinant Antibody (VS13-YC1237) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7999 | Anti-ZAP70 Immunohistochemistry Kit | IHC | |
| VS-0525-XY8000 | Anti-Mouse ZAP70 Immunohistochemistry Kit | IHC |
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