Mouse Anti-THPO Recombinant Antibody (clone TN1) (CAT#: PABZ-075)
Recombinant Mouse Antibody (TN1) is capable of binding to hTPO163, expressed in Chinese Hamster Ovary cells (CHO). hTPO163 interacts with soluble c-Mpl containing the extracellular cytokine receptor homology domains with 1:2 stoichiometry with the binding constants of 3. 3 × 109 M–1 and 1. 1 × 106 M–1.
Specific Inquiry
Figure 1 Dose-dependent inhibition of rhTPO-induced growth of FDCP-hMpl5 cells by the neutralizing Abs.
rhTPO (14.1 fmol/ml) was preincubated with various concentrations of each Ab. FDCPhMpl5 (1 × 104 cells/well) were then incubated with these mixtures. After three days, the MTS assay was performed. Symbols represent anti-HT1 (●), TN1 (▲) and PCF9 (■). Results are expressed as the mean ± SD of triplicate determinations. The absorbance of the maximal cell growth (without Ab) and the background (without factors) was 1.06 ± 0.05 and 0.47 ± 0.01, respectively.
Tahara, T., Kuwaki, T., Matsumoto, A., Morita, H., Watarai, H., Inagaki, Y., ... & Kato, T. (1998). Neutralization of biological activity and inhibition of receptor binding by antibodies against human thrombopoietin. Stem Cells, 16(1), 54-60.
Figure 2 Serum TPO levels and circulating platelet counts after myelosuppressive chemotherapy.
Three patients with acute myeloid leukemia in complete remission were treated with myelosuppressive chemotherapy. Serum TPO levels were measured by a sensitive ELISA (closed circles), and platelet counts were measured by an automated blood cell counter (open circles). The open triangles show the time of platelet transfusion (10 units). The arrows (↓) show the time of measurement of serum TPO levels and platelet counts just before and 1 h after platelet transfusion. (a) The first course of consolidation chemotherapy in patient 1 (course I). (b) The second course of consolidation chemotherapy in patient 1 (course II). (c) The third course of consolidation chemotherapy in patient 1 (course III). (d) The first course of maintenance/intensification chemotherapy in patient 2. (e) The first course of consolidation chemotherapy in patient 3.
Shinjo, K., Takeshita, A., Nakamura, S., Naitoh, K., Yanagi, M., Tobita, T., ... & Ohno, R. (1998). Serum thrombopoietin levels in patients correlate inversely with platelet counts during chemotherapy-induced thrombocytopenia. Leukemia, 12(3), 295.
Specifications
- Immunogen
- Human thrombopoietin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human THPO
- Species Reactivity
- Human
- Clone
- TN1
- Applications
- Neut, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody TN1 has been reported in applications of Neut, ELISA. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
ELISA The reported concentration of the antibody TN1 for ELISA detecting serum TPO was 10 μg/mL.
Target
- Alternative Names
- THPO; thrombopoietin; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1; MPL ligand; c-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin
- Gene ID
- 7066
- UniProt ID
- P40225
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-973 | Recombinant Anti-human THPO Antibody | WB, IF, FuncS | IgG |
| MHH-973 | Recombinant Human Anti-human THPO Antibody | ELISA, IP, FuncS | IgG |
| MOB-0207MC | Anti-Dog (Canine) Thrombopoietin Antibody | WB | |
| MOB-0208MC | Mouse Anti-THPO Recombinant Antibody (clone YC167) | WB, ICC, IHC-P, IHC-F, ELISA | Mouse IgG |
| PABW-122 | Human Anti-THPO Recombinant Antibody (clone TN1) | Neut | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-973-F(E) | Recombinant Anti-human THPO Antibody Fab Fragment | IF, FC, FuncS | Fab |
| MHH-973-F(E) | Recombinant Human Anti-human THPO Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| PFBZ-075 | Mouse Anti-THPO Recombinant Antibody (clone TN1); Fab Fragment | Neut, ELISA | Mouse Fab |
| PFBW-122 | Human Anti-THPO Recombinant Antibody (clone TN1); Fab Fragment | Neut | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-973-S(P) | Recombinant Anti-human THPO Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-973-S(P) | Recombinant Human Anti-human THPO Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| PSBZ-075 | Mouse Anti-THPO Recombinant Antibody (clone TN1); scFv Fragment | Neut, ELISA | Mouse scFv |
| PSBW-122 | Human Anti-THPO Recombinant Antibody (clone TN1); scFv Fragment | Neut | Human scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-178 | Recombinant Mouse Anti-Thrombopoietin Antibody (TN1) | WB, Neut, FuncS | IgG |
| PABX-178-S (P) | Recombinant Mouse Anti-Thrombopoietin Antibody scFv Fragment (TN1) | WB, Neut, FuncS | scFv |
| PABX-178-F (E) | Recombinant Mouse Anti-Thrombopoietin Antibody Fab Fragment (TN1 ) | WB, Neut, FuncS | Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3566 | Rabbit Anti-Thpo Recombinant Antibody (clone DS3566AB) | ELISA | Rabbit IgG |
| MOR-4456 | Rabbit Anti-THPO Recombinant Antibody (clone SI549DS) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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