Human Anti-ITGA4 Recombinant Antibody (clone HP1/2) (CAT#: PABL-360)
Recombinant Human Antibody (HP1/2) is capable of binding to α4 integrin, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-α4 integrin mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-α4 integrin mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Figure 1 Flow cytometry analysis of K562 α4β7 transfectants.
Wild type A4β7 and the indicated α4 mutant transfectants were incubated at 4 °C for 40 min with saturating concentrations of anti-α4β7 Act-1, anti-α4 HP1/2, anti-β1 TS2/16, or control P3 mAb. After washing, cells were incubated at 4 °C for 30 min with fluorescein isothiocyanate-conjugated secondary antibody (Dako, Glostrup, Denmark), and finally analyzed using a Coulter Epics XL flow cytometer.
Ruiz-Velasco, N., Guerrero-Esteo, M., Briskin, M. J., & Teixidó, J. (2000). The α4 integrin subunit Tyr187 has a key role in α4β7-dependent cell adhesion. Journal of Biological Chemistry, 275(10), 7052-7059.
Figure 2 Cell surface iodination and immunoprecipitation of α4β7 and α4β1 in K562 α4 transfectants.
Cells were labeled with 125I, solubilized, and the cell extracts immunoprecipitated with anti-α4β7 Act-1, anti-α4 HP1/2, anti-β1 TS2/16, or control P3 mAb. Immunoprecipitates were analyzed under non-reducing conditions by SDS-PAGE and autoradiography. The migration of the different forms of the α4 subunit and of the β1 and β7 subunits is indicated.
Ruiz-Velasco, N., Guerrero-Esteo, M., Briskin, M. J., & Teixidó, J. (2000). The α4 integrin subunit Tyr187 has a key role in α4β7-dependent cell adhesion. Journal of Biological Chemistry, 275(10), 7052-7059.
Specifications
- Immunogen
- Human integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human ITGA4
- Species Reactivity
- Human
- Clone
- HP1/2
- Applications
- FC, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ITGA4; integrin; alpha 4 (antigen CD49D; alpha 4 subunit of VLA-4 receptor); IA4; CD49D; integrin alpha-4; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D; alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor; alpha 4 subunit
- Gene ID
- 3676
- UniProt ID
- P13612
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-135 | Anti-Human Integrin alpha 4 beta 7 Recombinant Antibody (Etrolizumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L029G | anti-ITGA4 immunotoxin 15A8 (IgG)-Gel | Cytotoxicity assay, Functional assay |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-249 | Human Anti-ITGA4 Recombinant Antibody (clone MAb 21.6); scFv Fragment | Neut, FuncS | Human scFv |
| PSBL-360 | Human Anti-ITGA4 Recombinant Antibody (clone HP1/2); scFv Fragment | FC, ELISA, FuncS | Human scFv |
| HPAB-0466-YJ-S(P) | Human Anti-ITGA4 Recombinant Antibody (clone IN9A); scFv Fragment | ELISA, FC, Block | Human scFv |
| HPAB-1588WJ-S(P) | Mouse Anti-ITGA4 Recombinant Antibody; scFv Fragment (clone TA2) | ELISA, WB | Mouse scFv |
| HPAB-1620WJ-S(P) | Mouse Anti-ITGA4 Recombinant Antibody; scFv Fragment (clone HP1 / 2) | ELISA, WB | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-249 | Human Anti-ITGA4 Recombinant Antibody (clone MAb 21.6); Fab Fragment | Neut, FuncS | Human Fab |
| HPAB-0466-YJ-F(E) | Human Anti-ITGA4 Recombinant Antibody (clone IN9A); Fab Fragment | ELISA, FC, Block | Human Fab |
| HPAB-1588WJ-F(E) | Mouse Anti-ITGA4 Recombinant Antibody; Fab Fragment (clone TA2) | ELISA, WB | Mouse Fab |
| HPAB-1620WJ-F(E) | Mouse Anti-ITGA4 Recombinant Antibody; Fab Fragment (clone HP1 / 2) | ELISA, WB | Mouse Fab |
| HPAB-1621WJ-F(E) | Human Anti-ITGA4 Recombinant Antibody; Fab Fragment (clone huHP1 / 2) | ELISA, WB | Human Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-717LC | Anti-human ITGA4 Recombinant Antibody (IN 10) (TAB-717LC) | ELISA | Human antibody |
| TAB-717LC-S(P) | Anti-human ITGA4 scFv Fragment (IN 10) | ELISA | Human antibody |
| TAB-717LC-F(E) | Anti-human ITGA4 Fab Fragment (IN 10) | ELISA | Human antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-122 | Recombinant Mouse Anti-Integrin alpha 4 Antibody (ACT-1) | Neut, FuncS | IgG |
| PABX-122-S (P) | Recombinant Mouse Anti-Integrin alpha 4 Antibody scFv Fragment (ACT-1) | Neut, FuncS | scFv |
| PABX-122-F (E) | Recombinant Mouse Anti-Integrin alpha 4 Antibody Fab Fragment (ACT-1) | Neut, FuncS | Fab |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1549CQ | Recombinant Mouse Anti-ITGA4 Antibody (2B4) | BL | IgG1 |
| NEUT-1550CQ | Recombinant Rat Anti-Itga4 Antibody (9C10(MFR4.B)) | FC, BL, IHC | IgG2a, κ |
| NEUT-1551CQ | Recombinant Rat Anti-Itga4 Antibody (R1-2) | FC, IP, BL, IHC | IgG2b, κ |
| NEUT-1552CQ | Recombinant Rat Anti-Itga4 Antibody (PS/2) | FC, BL | IgG2b |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1553CQ | Recombinant Rat Anti-Itga4 Antibody (CBL914) | FC, Neut | IgG2a, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1873 | Hi-Affi™ Recombinant Rabbit Anti-ITGA4 Monoclonal Antibody (DS1873AB) | ICC/IF, IHC-P, IP, WB | IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-135 | Afuco™ Anti-ITGA4 ADCC Recombinant Antibody (Etrolizumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0083-YJ | Mouse Anti-ITGA4 Recombinant Antibody (clone HP2/1) | FC, FuncS, IHC-Fr, IP | Mouse IgG1 |
| ZG-0774J | Rabbit Anti-ITGA4 Recombinant Antibody (clone 1H4) | ELISA, WB | Rabbit IgG |
| VS3-XY898 | Mouse Anti-ITGA4 Recombinant Antibody, FITC (clone 4ABU49) | FC, IF | Mouse IgG1 |
| VS3-XY899 | Mouse Anti-ITGA4 Recombinant Antibody, PE (clone 4ABU49) | FC, IF | Mouse IgG1 |
| VS3-XY900 | Mouse Anti-ITGA4 Recombinant Antibody (clone 4ABU49) | FC, IF | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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