BV421-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer (CAT#: MHC-LC1327)
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of H2AFX-derived peptide of RIIPRHLQL covering 78-86 and HLA-G molecule. The pMHC tetramer recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg
(Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg
Figure 7 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg
Figure 8 Kidney
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg
Figure 10 Skin
(Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg
(Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AFX
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AFX
Specifications
- Allele
- HLA-G
- Class
- Class I
- MHC Species
- Human
- Antigen
- H2AFX
- Antigen Species
- Human
- Peptide
- RIIPRHLQL
- Range
- 78-86
- Conjugate
- BV421
- Application
- FCM
Target
- Antigen Introduction
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
- Alternative Names
- H2AFX; H2A histone family member X; H2AX; H2A.X; H2A/X
- Gene ID
- 3014
- UniProt ID
- P16104
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2435MZ | Recombinant Mouse Anti-Human H2AFX Antibody (clone 4G3) | ELISA, FC, ICC/IF, IHC-P, WB | Mouse antibody |
| ZG-0947F | Rabbit Anti-H2AFX Recombinant Antibody (ZG-0947F) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX492 | Mouse Anti-H2AFX Recombinant Antibody (clone 2A9-B5) | WB, IF | Mouse IgG2a |
| VS3-FY637 | Recombinant Mouse Anti-H2AFX Antibody (clone 4A2D5) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
| VS3-WK2167 | Rabbit Anti-H2AFX (pS139) Recombinant Antibody (clone R03-2Z1) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
Customer Reviews and Q&As
Customer Review
Q&As
High compatibility
The tetramer's compatibility with other fluorophores is a major advantage. It has streamlined our multi-parametric flow cytometry workflow, allowing for more comprehensive analyses.
Useful for sorting antigen-specific T cells
We found this tetramer particularly useful for sorting antigen-specific T cells for downstream RNA sequencing, providing clear insights into T cell reactivity across different cancer immunotherapies.
Reliable product
I was hesitant at first, however the high-throughput screening performance of this tetramer much exceeded our expectations. I've shortened some of my immunological assays because of its dependability.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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