Chymotrypsin Protease (TLCK treated), MS Grade (CAT#: Glyco-078CL)

Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.

  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 4 x 25 µg
  • Final Product
  • Peptide
  • Workflow Step
  • Protein Digestion
  • Detection Method
  • Mass Spectrometry
  • Starting Material
  • Cell Lysate, Protein samples
  • For Use With (Equipment)
  • Mass Spectrometer
  • Contents & storage
  • Store at -20°C in a nonfrost-free freezer.

Background

  • Description
  • This Chymotrypsin is a mass spectrometry (MS)-grade serine protease isolated from bovine pancreatic extracts. This chymotrypsin is TLCK-treated to eliminate tryptic activity. The selectivity of this enzyme is important for reproducible protein digestion and mass spectrometry-based protein identification. Chymotrypsin-digestion typically generates a larger number of shorter peptides compared to trypsin. This mass spectrometry-grade Chymotrypsin is packaged lyophilized (convenient 4 x 25 µg).
    The endoproteinase chymotrypsin specifically cleaves at the carboxyl side of tyrosine, phenylalanine, tryptophan and leucine. Two predominant forms of chymotrypsin, A and B, are found in equal amounts in bovine pancreas. They are similar proteins (80% homology), but have different proteolytic characteristics. Both forms of chymotrypsin are present in Chymotrypsin. Since trypsin may co-purify with Chymotrypsin derived from natural sources, Chymotrypsin has been treated with TLCK to eliminate potential tryptic activity, improving digestion specificity. Chymotrypsin can tolerate mild denaturing conditions, such as 0.1% SDS, 2M urea, 2M guanidine·HCl, 1% CHAPS, and 30% acetonitrile with optimal activity in the pH range 7.5 to 8.5. This lyophilized enzyme has a mass of 25 kDa and is stable for 1 year when stored at -20°C.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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