CBGlyco™ Direct IP Kit (CAT#: Glyco-038CL)

Direct IP Kit uses an activated resin to covalently immobilize IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.

The primary benefits resulting from this method are the opportunity to use any species or subclass of purified antibody (not just types that bind to Protein A or G) and the ability to purify target protein without contamination by the antibody. The method also makes it possible to immunoprecipitate antigens from serum samples without co-purifying non-target immunoglobulins. Finally, the kit uses microcentrifuge spin cups to effectively wash and separate samples from the beaded agarose resin.

  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 50 reactions
  • Technique
  • Immunoprecipitation (IP), Pull-Down
  • Assay Type
  • Affinity Purification
  • Target Specificity
  • Not Target-Specific
  • For Use With (Equipment)
  • Microcentrifuge
  • Contents & storage
  • Sufficient For: Preparing and performing at least 50 IP reactions, each with 10 µL of resin
    • Aminolink Plus Coupling Resin, 2 mL
    • Coupling Buffer (20X), 25 mL
    • Quenching Buffer, 50 mL
    • Wash Solution, 50 mL
    • Sodium Cyanoborohydride Solution (5M), 0.5 mL
    • IP Lysis/Wash Buffer, 2 x 50 mL
    • Conditioning Buffer (100X), 5 mL
    • Tris-Buffered Saline (20X), 25 mL
    • Elution Buffer, 50 mL
    • Lane Marker Sample Buffer (5X), 5 mL
    • Control Agarose Resin, 2 mL
    • Spin Columns and Collection Tubes

    Store at 4°C.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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