Anti-IAV HA Recombinant Antibody (TAB-027ML) (CAT#: TAB-027ML)
Recombinant monoclonal antibody to Influenza A virus hemagglutinin HA. It is a human monoclonal antibody that can be potentially used in the treatment of Influenza B infection.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-IAV HA Recombinant Antibody (TAB-027ML) in SDS-PAGE
SDS-PAGE analysis of TAB-027ML in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 2 Anti-IAV HA Recombinant Antibody (TAB-027ML) in SEC-HPLC
The purity of TAB-027ML was 97.661% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Anti-IAV HA Recombinant Antibody (TAB-027ML) in ELISA
ELISA analysis of TAB-027ML was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with TAB-027ML. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-IAV HA Recombinant Antibody (TAB-027ML) in ELISA
ELISA analysis of TAB-027ML was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with TAB-027ML. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 5 Anti-IAV HA Recombinant Antibody (TAB-027ML) in ELISA
ELISA analysis of TAB-027ML was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with TAB-027ML. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 6 Anti-IAV HA Recombinant Antibody (TAB-027ML) in ELISA
ELISA analysis of TAB-027ML was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein (His Tag). Then blocked with BSA and incubated with TAB-027ML. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 7 Anti-IAV HA Recombinant Antibody (TAB-027ML) in WB
Western blot analysis of TAB-027ML was performed with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-027ML and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.3 μg)
Lane 2: Reduced antigen (0.6 μg)
Figure 8 Anti-IAV HA Recombinant Antibody (TAB-027ML) in WB
Western blot analysis of TAB-027ML was performed with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-027ML and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.3 μg)
Lane 2: Reduced antigen (0.6 μg)
Figure 9 Anti-IAV HA Recombinant Antibody (TAB-027ML) in WB
Western blot analysis of TAB-027ML was performed with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-027ML and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.3 μg)
Lane 2: Reduced antigen (0.6 μg)
Figure 10 Anti-IAV HA Recombinant Antibody (TAB-027ML) in DB
Dot Blot analysis of TAB-027ML was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
TAB-027ML incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
Figure 11 Anti-IAV HA Recombinant Antibody (TAB-027ML) in DB
Dot Blot analysis of TAB-027ML was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
TAB-027ML incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
Figure 12 Anti-IAV HA Recombinant Antibody (TAB-027ML) in DB
Dot Blot analysis of TAB-027ML was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
TAB-027ML incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
Figure 13 Anti-IAV HA Recombinant Antibody (TAB-027ML) in DB
Dot Blot analysis of TAB-027ML was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein (His tag).
TAB-027ML incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1, κ
- Specificity
- Influenza A virus hemagglutinin HA
- Species Reactivity
- Influenza A virus
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies
- Conjugate
- Unconjugated
- Related Disease
- Influenza A infection
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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NABG-078 | Recombinant Anti-IAV HA VHH Single Domain Antibody | FC, CA, IP, FuncS | Llama VHH |
HPAB-0344CQ | Recombinant Llama Anti-IAV HA Single Domain Antibody (HPAB-0344CQ) | ELISA, Neut | Llama VHH |
HPAB-0345CQ | Recombinant Llama Anti-IAV HA Single Domain Antibody (HPAB-0345CQ) | ELISA, Neut | Llama VHH |
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