CBGlyco™ Glycoprotein Carbohydrate Estimation Kit (CAT#: Glyco-059CL)
Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.
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Specifications
- Product Size
- 500 mg
- Detection Method
- Colorimetric
- Technique
- Absorbance, Solution-based Detection
- Assay Type
- Protein Quantitation Assay
- For Use With (Equipment)
- Microplate Reader, Spectrophotometer
- Contents & storage
- Sufficient For: 60 tube assays or 250 microplate assays
• Sodium meta-Periodate, 500 mg
• Glycoprotein Detection Reagent, 500 mg
• Glycoprotein Assay Buffer, 250 mL
• Glycoprotein Standards Set, 5-protein set
Background
- Description
- The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (percent carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550 nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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